Evaluation of Inoculation Method of Citrus Yellow Vein Clearing Virus and Identification of Resistance of Main Cultivars

doi:10.11924/j.issn.1000-6850.casb2020-0208

Abstract

Abstract:

At present, there is no effective method to prevent and cure Citrus yellow vein clearing virus (CYVCV), which has a fast spreading speed and serious harm. The determination of inoculation transmission intensity, and the planting and breeding of resistant varieties can provide an important basis for the prevention and control of CYVCV. In this study, CYVCV was inoculated into 6 different Citrus varieties by grafting, juice friction and injection to determine the infection intensity, and determine the resistance of the varieties to CYVCV. The test results showed that: (1) the grafting morbidity rate and disease index were the highest, reaching 87.5% and 40.27, in addition, the affected plants showed typical symptoms of CYVCV, with severe yellowing, severe yellow vein and shrinkage, and leaf curling; the incidence rate and disease index of injection were 41.67% and 18.05, and the incidence rate of juice friction and disease index were low, 4.17% and 1.39, respectively, showing that CYVCV was a kind of citrus virus disease with high infectivity of grafting; (2) after inoculation of CYVCV, the incidence and disease index of ‘Yulik’ reached 83.33% and 52.78, respectively, its yellowing symptom was serious, had more serious yellow vein, and slight curl and crinkle. The results of the determination of resistance to CYVCV showed that the medium resistance to CYVCV was found in ‘Harumi’, ‘Tacopon’, ‘Daya’, ‘MingRijian’; ‘Sugar orange’ was S; ‘Yulik’ was HS. This study can provide reference for the early detection, prevention and control of citrus yellow vein disease, and the cultivation and disease resistance breeding of citrus.

Key words: Citrus, CYVCV, inoculate, grafting transmission, resistance, ‘Yulik’

CLC Number:

S432.4+1

Fan Zhonghan, Hu Rongping, Chen Qinghua, Chen Qingdong, Li Honghao. Evaluation of Inoculation Method of Citrus Yellow Vein Clearing Virus and Identification of Resistance of Main Cultivars[J]. Chinese Agricultural Science Bulletin, 2020, 36(31): 110-114.

Figures/Tables 7

References 27

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序号	药剂名称	生产厂家
1	磷酸氢二钾	成都市科隆化学品有限公司
2	亚硫酸钠	成都金山化学试剂有限公司
3	三羟甲基甲胺	淮安和元化工有限公司

序号	药剂名称	生产厂家
1	磷酸氢二钾	成都市科隆化学品有限公司
2	亚硫酸钠	成都金山化学试剂有限公司
3	三羟甲基甲胺	淮安和元化工有限公司

级别	代表值	发病枝条比例或黄化、脉明、反卷占全树比例(x)
I	0	0
II	1	x<1/3
III	2	1/3≤x<2/3
IV	3	x≥2/3（至整株死亡）

级别	代表值	发病枝条比例或黄化、脉明、反卷占全树比例(x)
I	0	0
II	1	x<1/3
III	2	1/3≤x<2/3
IV	3	x≥2/3（至整株死亡）

级别	抗病性	相对抗性指数(x)
0	免疫(I)	x≥1
1	高抗(HR)	0.90≤x<1
3	抗病(R)	0.80≤x<0.90
5	中抗(MR)	0.50≤x<0.80
7	感病(S)	0.20≤x<0.5
9	高感(HS)	x<0.20