Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2021, Vol. 37 ›› Issue (16): 150-155.doi: 10.11924/j.issn.1000-6850.casb2020-0429

Special Issue: 生物技术 园艺

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Application of Pressurized Solvent Extraction Technology in the Extraction of Polysaccharide from Two Kinds of Edible and Medicinal Fungi

Yan Jiajia1,2(), Wan Lu1,2, Wu Tong1,3(), Zheng Chunying1,2()   

  1. 1Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin 150500
    2Key Laboratory of Microbiology, College of Heilongjiang Province/ School of Life Sciences, Heilongjiang University, Harbin 150080
    3Key Laboratory of Molecular Biology, College of Heilongjiang Province/ School of Life Sciences, Heilongjiang University, Harbin 150080
  • Received:2020-09-07 Revised:2021-01-18 Online:2021-06-05 Published:2021-06-16
  • Contact: Wu Tong,Zheng Chunying E-mail:2424643682@qq.com;523791254@qq.com;zhengchunying68@163.com

Abstract:

The paper aims to develop Pressurized Solvent Extraction (PSE) for extracting polysaccharides in two kinds of edible and medicinal fungi with the Polyporus umbellatus and Poria cocos were chosen as raw materials, the single factor and orthogonal experiments were used to explore the optimal conditions for the extraction of polysaccharide by PSE. The results showed that the optimized parameters of PSE employed temperature 110℃, pressure 110 bar, and extracting time was set at 20 min for 2 times to extract polysaccharides in Polyporus umbellatus completely. Under the condition, the content of Polyporus umbellatus polysaccharide was (7.8684±0.1096) mg/g. Meanwhile, the optimized parameters of PSE employed temperature 110℃, pressure 120 bar, and extracting time was set at 25 min for 2 times to extract Poria cocos polysaccharides completely. Under the condition, the content of Poria cocos polysaccharide was (8.6965±0.1087) mg/g. As a conclusion, PSE can be used to extract polysaccharides from the two kinds of edible and medicinal fungi quickly and accurately.

Key words: PSE, Polyporus umbellatus polysaccharides, Poria cocos polysaccharides, extraction technology, single factor experiment, orthogonal experiment

CLC Number: