Active Ingredients in Picoxystrobin Suspension Concentrate and Water Dispersible Granule: Determination by HPLC

doi:10.11924/j.issn.1000-6850.casb2021-0125

Abstract

Abstract:

In order to establish a general method to determine active ingredient in picoxystrobin suspension concentrate (SC) and water dispersible granule (WG) using the same high performance liquid chromatography (HPLC) condition,the samples were dissolved with acetonitrile, and analyzed by RP-HPLC. The mobile phase was acetonitrile-water (volume ratio 80: 20), the flow rate was 1.0 mL/min and the amount of injection was 5.0 μL. Separation was achieved on the Symmetry-C₁₈ column and diode array detector (DAD) at 220 nm, and the quantitative result was calculated by external standard method. In addition, the peak purity was checked through DAD and the method was tested by different laboratories. The result showed good linearities in the concentration ranged from 100.1 mg/L to 1000.8 mg/L, with the correlation coefficient (R²) of 0.9993. The recovery of SC and WG were 100.64% and 99.74%, respectively. The RSD of measurement results were 1.31% and 0.69% respectively. RSD of different laboratories were 0.8052% and 0.6003%. RSD_R were 0.8560% and 1.5625%, respectively. The precision, accuracy and peak purity results meet the requirements of analysis for pesticide products. The method is suitable for the determination active ingredient for picoxystrobin in suspension concentrate and water dispersible granule.

Key words: picoxystrobin, suspension concentrate, water dispersible granule, HPLC, active ingredient

CLC Number:

Ma Tengfei, Jia Sunyue, Liu Lin, Zhou Qin, Gao Jinsheng. Active Ingredients in Picoxystrobin Suspension Concentrate and Water Dispersible Granule: Determination by HPLC[J]. Chinese Agricultural Science Bulletin, 2021, 37(27): 117-124.

Figures/Tables 11

References 26

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编号	称样质量/g	啶氧菌酯质量分数/%	最大值/%	最小值/%	平均值/%	标准偏差/%	相对标准偏差(RSD)/%
STD-SC-A	0.05127	-	23.08	22.34	22.74	0.29	1.31
SC-A1	0.22201	22.64
SC-A2	0.22202	22.34
SC-A3	0.22298	23.08
SC-A4	0.22219	22.99
SC-A5	0.22278	22.67

编号	称样质量/g	啶氧菌酯质量分数/%	最大值/%	最小值/%	平均值/%	标准偏差/%	相对标准偏差(RSD)/%
STD-SC-A	0.05127	-	23.08	22.34	22.74	0.29	1.31
SC-A1	0.22201	22.64
SC-A2	0.22202	22.34
SC-A3	0.22298	23.08
SC-A4	0.22219	22.99
SC-A5	0.22278	22.67

编号	称样质量/g	啶氧菌酯质量分数/%	最大值/%	最小值/%	平均值/%	标准偏差/%	相对标准偏差(RSD)/%
STD-WG-A	0.05005	-	50.99	50.10	50.53	0.35	0.69
WG-A1	0.09813	50.99
WG-A2	0.09817	50.37
WG-A3	0.09819	50.79
WG-A4	0.09921	50.10
WG-A5	0.09867	50.42

编号	称样质量/g	啶氧菌酯质量分数/%	最大值/%	最小值/%	平均值/%	标准偏差/%	相对标准偏差(RSD)/%
STD-WG-A	0.05005	-	50.99	50.10	50.53	0.35	0.69
WG-A1	0.09813	50.99
WG-A2	0.09817	50.37
WG-A3	0.09819	50.79
WG-A4	0.09921	50.10
WG-A5	0.09867	50.42

溶液名称	试样质量/g	标样质量/g	实测质量/g	回收率/%	平均回收率/%
STD-B	-	0.05005	-	-	100.64
SC-B1	0.11109	0.02519	0.05031	100.73
SC-B2	0.11102	0.02516	0.05041	100.01
SC-B3	0.11124	0.02526	0.04930	98.51
SC-B4	0.11101	0.02501	0.05145	103.47
SC-B5	0.11134	0.02524	0.04984	99.57