Research Progress of SSR Molecular Markers of Dendrobium Plants

doi:10.11924/j.issn.1000-6850.casb2021-0338

Abstract

Abstract:

The study reviewed research progress of Dendrobium plants in the fields such as genetic diversity analysis, relationship comparison, fingerprint and genetic linkage map construction, variety identification, and germplasm purity identification, analyzed main problems of SSR molecular markers in the research of Dendrobium plants, and proposed that the future development of SSR molecular markers should be aimed at more groups and varieties of Dendrobium, adopt the latest third-generation sequencing technology to mine trait-associated markers, and formulate the unified national or industrial standards for variety identification markers, thus providing more reference for the in-depth application of SSR molecular marks in Dendrobium.

Key words: Dendrobium, SSR, molecular marker, genetic diversity, research progress

CLC Number:

S567

PENG Chan, ZHANG Xinye, LIU Zongkun, MA Linjiang, CHEN Huiling. Research Progress of SSR Molecular Markers of Dendrobium Plants[J]. Chinese Agricultural Science Bulletin, 2022, 38(13): 36-40.

References 54

[1]	NIU Z T, PAN J J, XUE Q Y, et al. Plastome-wide comparison reveals new SNV resources for the authentication of Dendrobium huoshanense and its corresponding medicinal slice (Huoshan Fengdou)[J]. Acta Pharmaceutica sinica B, 2018, 8(3):466-477. doi: 10.1016/j.apsb.2017.12.004 URL
[2]	李清, 李标, 郭顺星. 兰科石斛属植物分子生物学研究进展[J]. 中国中药杂志, 2016, 41(15):2753-2761.
[3]	廖格, 张友源, 宋希强, 等. 观赏石斛与药用石斛HPLC指纹图谱及生物活性的比较[J]. 热带生物学报, 2015, 6(4):420-425.
[4]	ZHANG X Q, GAO J Y. In vitro tetraploid induction from multigenotype protocorms and tetraploid regeneration in Dendrobium officinale[J]. Plant cell, tissue and organ culture, 2020, 141(2):289-298. doi: 10.1007/s11240-020-01786-6 URL
[5]	王元成, 曾艺芸, 李振坚, 等. 细叶石斛和翅梗石斛花朵赋香成分的GC-MS分析[J]. 林业科学研究, 2020, 33(3):116-123.
[6]	郑宝强, 朱胜蕾, 李奎, 等. 中国原生种石斛兰的育种价值分析[J]. 北京林业大学学报, 2018, 40(4):102-108.
[7]	母多. 六种石斛的鉴别研究与质量评价[D]. 云南: 昆明医科大学, 2018:7-8.
[8]	KUANG M T, LI J Y, YANG X B, et al. Structural characterization and hypoglycemic effect via stimulating glucagon-like peptide-1 secretion of two polysaccharides from Dendrobium officinale[J]. Carbohydrate polymers, 2020,241.
[9]	王云威, 王景雪. 铁皮石斛多糖对2型糖尿病小鼠降糖降脂的作用[J]. 食品科学, 2020, 21:127-132.
[10]	CHEN H, NIE Q, HU J, et al. Metabolism amelioration of Dendrobium officinale polysaccharide on type II diabetic rats[J]. Food hydrocolloids, 2019, 102:105582. doi: 10.1016/j.foodhyd.2019.105582 URL
[11]	HUANG S, WU Q, LIU H, et al. Alkaloids of Dendrobium nobile Lindl. Altered hepatic lipid homeostasis via regulation of bile acids[J]. Journal of Ethnopharmacology, 2019, 241:111976-111976. doi: 10.1016/j.jep.2019.111976 URL
[12]	CHANG J Z, ZHOU Y T, CONG G B, et al. Dendrobium candidum protects against diabetic kidney lesions through regulating vascular endothelial growth factor, Glucose Transporter 1, and connective tissue growth factor expression in rats[J]. Journal of cellular biochemistry, 2019, 120(8):13924-13931.. doi: 10.1002/jcb.28666 URL
[13]	颜美秋, 苏洁, 俞静静, 等. 铁皮石斛醇提取物对复合饮食因素所致代谢性高血压大鼠的作用及物质基础研究[J]. 中国中药杂志, 2019, 44(22):4896-4904.
[14]	罗颖懿. 新西兰牡荆苷Ⅱ促肝癌凋亡与抑制肺癌EMT的机制研究[D]. 广州: 广州中医药大学, 2019:54-57.
[15]	ZHANG S, TU H Y, ZHU J M, et al. Dendrobium nobile Lindl. polysaccharides improve follicular development in PCOS rats[J]. International journal of biological macromolecules, 2020, 149:826-834. doi: 10.1016/j.ijbiomac.2020.01.196 URL
[16]	食品安全标准与监测评估司. 关于对党参等9种物质开展按照传统既是食品又是中药材的物质管理试点工作的通知[EB/OL]. http://www.nhc.gov.cn/sps/s7885/202001/1ec2cca04146450d9b14acc2499d854f.shtml
[17]	TIKENDRA L, AMOM T, NONGDAM P. Molecular genetic homogeneity assessment of micropropagated Dendrobium moschatum Sw.- A rare medicinal orchid, using RAPD and ISSR markers[J]. Plant gene, 2019, 19:100196. doi: 10.1016/j.plgene.2019.100196 URL
[18]	崔学强, 唐璇, 黄昌艳, 等. 22种石斛兰遗传多样性分析及DNA指纹图谱构建[J]. 分子植物育种, 2020, 19(9):10.
[19]	杨贞, 蔡友铭, 张永春, 等. 基于SRAP分子标记的铁皮石斛遗传多样性分析[J]. 上海农业学报, 2019, 35(5):23-27.
[20]	ZHAO T M, ZHENG S G, HU Y D, et al. Classification of interspecific and intraspecific species by genome-wide SSR markers on Dendrobium[J]. South African journal of botany, 2019, 127:136-146. doi: 10.1016/j.sajb.2019.08.051 URL
[21]	张君毅, 吴永辉, 刘嘉, 等. 利用TRAP和SCAR标记评价铁皮石斛的遗传多样性与抗寒性[J]. 植物生理学报, 2020, 56(4):743-751.
[22]	HE B B, GENG R M, CHENG L R, et al. Genetic diversity and fingerprinting of 33 standard flue-cured tobacco varieties for use in distinctness, uniformity, and stability testing[J]. BMC plant biology, 2020, 20(1):378. doi: 10.1186/s12870-020-02596-w URL
[23]	HUANG C J, CHU F H, HUANG Y S, et al. Development and technical application of SSR-based individual identification system for Chamaecyparis taiwanensis against illegal logging convictions[J]. Scientific reports, 2020, 10(1):22095. doi: 10.1038/s41598-020-79061-z URL
[24]	GU S, DING X Y, WANG Y, et al. Isolation and characterization of microsatellite markers in Dendrobium officinale, an endangered herb endemic to China[J]. Molecular ecology notes, 2007, 7(6):1166-1168. doi: 10.1111/j.1471-8286.2007.01817.x URL
[25]	FAN W J, LUO Y M, LI X X, et al. Development of microsatellite markers in Dendrobium fimbriatum Hook, an endangered Chinese endemic herb[J]. Molecular ecology resources, 2009, 9(1):373-375. doi: 10.1111/j.1755-0998.2008.02456.x URL
[26]	CAI X Y, FENG Z Y, HOU B W, et al. Development of microsatellite markers for genetic diversity analysis of Dendrobium loddigesii Rolfe, an endangered orchid in China[J]. Biochemical systematics and ecology, 2012, 43:42-47. doi: 10.1016/j.bse.2011.09.003 URL
[27]	YAN W, HOU B, XUE Q, et al. Different evolutionary processes in shaping the genetic composition of Dendrobium nobile in southwest China[J]. Genetica, 2015, 143(3):361-371. doi: 10.1007/s10709-015-9835-4 URL
[28]	郑基阳, 陈乃富, 王晖, 等. 霍山石斛微卫星标记的筛选及遗传多样性分析[J]. 中国中药杂志, 2011, 36(21):2926-2931.
[29]	WANG H, CHEN N F, ZHENG J Y, et al. Isolation and characterization of eleven polymorphic microsatellite loci for the valuable medicinal plant Dendrobium huoshanense and cross-species amplification[J]. International journal of molecular sciences, 2012, 13(12):16779-16784. doi: 10.3390/ijms131216779 URL
[30]	HOU B W, LUO J, DING X. Genetic diversity assessment and ex situ conservation strategy of the endangered Dendrobium officinale (Orchidaceae) using new trinucleotide microsatellite markers[J]. Plant systematics and evolution, 2012, 298:1483-1491. doi: 10.1007/s00606-012-0651-3 URL
[31]	袁英惠. 利用微卫星标记分析球花石斛的遗传多样性并鉴定其药材的地理来源[D]. 南京: 南京师范大学, 2011:41-42.
[32]	蔡小彦. 运用SRAP和SSR分子标记研究粉花石斛的遗传多样性和居群遗传结构[D]. 南京: 南京师范大学, 2011:42-44.
[33]	徐蕾, 刘莉, 彭少丹, 等. 利用SSR标记研究铁皮石斛的遗传多样性[J]. 分子植物育种, 2015, 13(7):1616-1622.
[34]	肖冬长, 张智俊, 管雨. 铁皮石斛微卫星SSR设计与应用[J]. 生物技术通报, 2012(7):88-92.
[35]	赵瑞强. 基于分子标记的铁皮石斛种质资源遗传多样性研究[D]. 杭州: 浙江农林大学, 2012:55-56.
[36]	董晓曼, 袁媛, 查良平, 等. 滇皖产区铁皮石斛居群SSR分子身份证的构建[J]. 中国现代中药, 2017, 19(5):617-624.
[37]	余文霞, 雷胄熙, 袁媛, 等. 铁皮石斛种质资源DNA身份证的构建及遗传相似性分析[J]. 中国实验方剂学杂志, 2019, 25(1):16-21.
[38]	张雅琼, 黄莉, 申毓晗, 等. 铁皮石斛变异体的差异性分析[J]. 西南农业学报, 2015, 28(3):1236-1241.
[39]	汪尚. 铁皮石斛种质资源遗传多样性评价及其遗传连锁图谱的构建[D]. 杭州: 杭州师范大学, 2012:44-45.
[40]	潘宏兵. 石斛遗传连锁图谱构建[D]. 海口: 海南大学, 2013:40-41.
[41]	任羽. 石斛兰种质资源评价、创新及遗传图谱构建的研究[D]. 海口: 海南大学, 2013:79-80.
[42]	刘士辉, 李怀志, 沈若刚, 等. 石斛种质资源遗传多样性的SSR分析及指纹图谱构建[J]. 浙江农业科学, 2017, 58(7):1183-1185.
[43]	张志勇, 周美玲, 梁金平, 等. 冠豸山铁皮石斛种质资源遗传多样性分析[J]. 分子植物育种, 2019, 17(18):6179-6185.
[44]	赵庭梅, 郑世刚, 胡亚东, 等. 基因组SSR应用于铁皮、迭鞘和金钗石斛种内的遗传分析[J]. 分子植物育种, 2020, 11(18):3673-3682.
[45]	任羽, 王呈丹, 徐世松, 等. 石斛兰亲缘关系的SSR分析[J]. 热带作物学报, 2013, 34(7):1252-1256.
[46]	韩晓霞, 章静钢, 张鹏博, 等. 基于SSR的铁皮石斛实生群体遗传多样性研究[J]. 江苏农业科学, 2016, 44(6):90-93.
[47]	章竞子. Real-time PCR及SSR技术在齿瓣石斛药材鉴别中的应用[D]. 南京: 南京师范大学, 2013:49-52.
[48]	谢明璐, 侯北伟, 韩丽, 等. 珍稀铁皮石斛SSR标记的开发及种质纯度鉴定[J]. 药学学报, 2010, 45(5):667-672.
[49]	丁鸽, 张代臻, 丁小余. 晶帽石斛多态性微卫星开发及种质鉴定[J]. 贵州农业科学, 2015, 43(10):170-172.
[50]	谢小波, 孙崇波, 宋仙水. 铁皮石斛γ射线诱变及后代变异研究[J]. 浙江农业科学, 2016, 57(5):687-690.
[51]	张志勇, 周美玲, 梁金平, 等. 铁皮石斛新品种福斛1号的选育. 福建农业学报, 2020, 35(7):709-716.
[52]	李永清, 江金兰, 叶炜, 等. SSR分子标记鉴定铁皮石斛与霍山石斛的正反交后代[J]. 福建农业学报, 2017, 32(8):870-873.
[53]	刘玉洋, 徐靖, 何金宇, 等. 铁皮石斛和金钗石斛杂交后代的分子鉴定[J]. 浙江农业科学, 2018, 59(9):1709-1713.
[54]	王培育, 郑世仲, 叶炜, 等. 铁皮石斛与金钗石斛杂交及回交后代的SSR鉴定[J]. 亚热带农业研究, 2019, 15(4):251-256.