Identification and Expression Analysis of MRS2/MGT Gene Family in Tobacco

doi:10.11924/j.issn.1000-6850.casb2021-0793

Abstract

Abstract:

To identify magnesium transporter (MGT) gene in tobacco genome and explore the mechanism of magnesium ion transport by MGT in tobacco plant, seven NtMGTs with conservative Gly-Met-Asn tripeptide motifs were identified from tobacco genome by homologous sequence alignment using the magnesium transporter family genes in rice and Arabidopsis genomes as reference sequences. The results showed that the expression of NtMGTs was tissue-specific and light-induced under different magnesium supply levels and light intensities. The expression of NtMGT1 in roots and NtMGT2, NtMGT4 and NtMGT5 in leaves of tobacco plants increased under strong light intensity. With the increase of magnesium supply, the expression of NtMGT1 gene in the root system increased, which was consistent with the change trend of magnesium content in the root system, suggesting that NtMGT1 gene mainly mediated the absorption of magnesium in the root system of tobacco plant. The expression levels of NtMGT2, NtMGT4 and NtMGT5 genes in leaves increased first and then decreased, indicating that the three genes belonged to a high affinity magnesium ion transport system. These results implied that under the stress of high temperature and strong light intensity, appropriate magnesium supply could increase the expression of NtMGT1 in the root part of tobacco plants, promote the absorption of magnesium ion by the root system, enhance the gene expression of NtMGT2, NtMGT4 and NtMGT5 in shoot part of tobacco plants, promote the transport of magnesium ion, and ensure the normal growth and development of tobacco plants. The results of this study can provide theoretical guidance for reasonable application of magnesium in tobacco production.

Key words: tobacco, magnesium supply, magnesium transporter protein, MRS2/MGT gene family, strong light intensity and high temperature stress

CLC Number:

Q94
S572

WU Ti, WEI Xiaoling, FENG Changqing, HUANG Yunxia, XU Shichang, QIU Fuxiang, ZHENG Yingjie, LI Wenqing, HE Huaqin. Identification and Expression Analysis of MRS2/MGT Gene Family in Tobacco[J]. Chinese Agricultural Science Bulletin, 2022, 38(16): 18-26.

Figures/Tables 9

References 26

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基因名	上游引物(5'-3')	下游引物(5'-3')
NtMGT1	GGTTGCCATCTTCGGGGTT	AGCGCTTATTTCCCTCATAGGCTC
NtMGT2	GCAGCCGATATACTCCGACCAAAC	ATGACCGTATTCCCGTCCCCTTC
NtMGT4	GTGGATCAGGTGCAGCCAGTTG	TGCCCGAAGTAAGCCTCCAATAAC
NtMGT5	ATGGGTGGTAATCATTAGTGCTATC	CTTGGATGCTGTATGGTTCCTTTC
β-actin	GATCTTGCTGGTCGTGATCT	ACTTCCGGACATCTGAACCT

基因名	上游引物(5'-3')	下游引物(5'-3')
NtMGT1	GGTTGCCATCTTCGGGGTT	AGCGCTTATTTCCCTCATAGGCTC
NtMGT2	GCAGCCGATATACTCCGACCAAAC	ATGACCGTATTCCCGTCCCCTTC
NtMGT4	GTGGATCAGGTGCAGCCAGTTG	TGCCCGAAGTAAGCCTCCAATAAC
NtMGT5	ATGGGTGGTAATCATTAGTGCTATC	CTTGGATGCTGTATGGTTCCTTTC
β-actin	GATCTTGCTGGTCGTGATCT	ACTTCCGGACATCTGAACCT

基因名称	基因编号	转录编号	蛋白编号
NtMGT1	107824590	XM_016651392.1	XP_016506878.1
NtMGT2	107797716	XM_016620625.1	XP_016476111.1
NtMGT3	107775786	XM_016595567.1	XP_016451053.1
NtMGT4	107765702	XM_016584374.1	XP_016439860.1
NtMGT5-1	107778350	XM_016598596.1	XP_016454083.1
NtMGT5-2	107778350	XM_016598597.1	XP_016454082.1
NtMGT6	107825361	XM_016652209.1	XP_016507695.1
NtMGT7	107791732	XM_016613852.1	XP_016469338.1

基因名称	基因编号	转录编号	蛋白编号
NtMGT1	107824590	XM_016651392.1	XP_016506878.1
NtMGT2	107797716	XM_016620625.1	XP_016476111.1
NtMGT3	107775786	XM_016595567.1	XP_016451053.1
NtMGT4	107765702	XM_016584374.1	XP_016439860.1
NtMGT5-1	107778350	XM_016598596.1	XP_016454083.1
NtMGT5-2	107778350	XM_016598597.1	XP_016454082.1
NtMGT6	107825361	XM_016652209.1	XP_016507695.1
NtMGT7	107791732	XM_016613852.1	XP_016469338.1

基因	基因编号	氨基酸数目	分子量/kDa	结构域	等电位点	膜螺旋结构数	亚细胞定位
NtMGT1	107824590	445	50.54	Mrs2_Mfm1p-like	5.20	2	Plasma membrane
NtMGT2	107797716	499	54.95	Mrs2_Mfm1p-like	4.83	2	Chloroplast
NtMGT3	107775786	499	54.97	Mrs2_Mfm1p-like	4.83	2	Chloroplast
NtMGT4	107765702	393	44.42	Mrs2_Mfm1p-like	5.10	3	Plasma membrane
NtMGT5	107778350	393	44.42	Mrs2_Mfm1p-like	5.10	3	Plasma membrane
NtMGT6	107825361	372	41.71	Mrs2_Mfm1p-like	4.87	2	Chloroplast
NtMGT7	107791732	442	49.84	Mrs2_Mfm1p-like	6.09	2	Plasma membrane