Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2022, Vol. 38 ›› Issue (22): 157-164.doi: 10.11924/j.issn.1000-6850.casb2021-0779

Special Issue: 生物技术

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Polysaccharide ASPF from Acanthopanax senticosus Fructification: Structural Characterization and Its Anti-lung Cancer Activity in Vitro

CUI Xuejiao1(), TONG Xiaoyu2, ZHANG Yanlong1,2(), ZENG Weimin1,2()   

  1. 1School of Life Sciences, Heilongjiang University/ Heilongjiang Provincial Key Laboratory of Microbiology, Harbin 150080
    2Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education/ Heilongjiang University, Harbin 150500
  • Received:2021-08-13 Revised:2022-03-12 Online:2022-08-05 Published:2022-08-22
  • Contact: ZHANG Yanlong,ZENG Weimin E-mail:cuixuejiao07@163.com;ylzhanghd@163.com;2002081@hlju.edu.cn

Abstract:

Homogenous molecular weight polysaccharide ASPF was purified from the fructification of Acanthopanax senticosus, and its structure characterization and in vitro anti-lung cancer activity were explored. The extracted crude polysaccharides were separated by DEAE-52 column chromatography to obtain 3 components, after screening for in vitro anti-lung cancer activity, ASPB-3 was obtained by Sephadex G-200 gel column chromatography to get ASPF. GPC, IR and HPLC methods were selected to analyze the structure of the polysaccharide, and the CCK-8 method was used to study its effect on the survival rate of lung cancer cells in vitro. The content of uronic acid in ASPF was 22.9%. It was an acidic pectin-like structure polysaccharide. Its relative molecular mass MW was 9.7462×104. IR scanning results showed that it had a pyran ring structure, which was composed of rhamnose and galacturonic acid. The results of monosaccharide determination showed that it was composed of six monosaccharides: rhamnose, galacturonic acid, glucose, xylose, galactose, and arabinose. There was an inhibitory effect on A549 cells, and its mechanism might be related to the effect on the apoptosis gene Bcl-2/Bax. In summary, ASPF has a certain in vitro inhibitory effect on human lung cancer cells.

Key words: Acanthopanax senticosus fructification, polysaccharide, purification, structural characterization, anti-lung cancer

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