Chinese Agricultural Science Bulletin ›› 2022, Vol. 38 ›› Issue (25): 143-150.doi: 10.11924/j.issn.1000-6850.casb2021-0888
Previous Articles Next Articles
ZHANG He1,2, YOU Mengyao1,2, WAN Lu1,2, YAN Jiajia1,2, LIU Songmei1,2(), ZHENG Chunying1,2()
Received:
2021-09-16
Revised:
2022-02-10
Online:
2022-09-05
Published:
2022-08-26
Contact:
LIU Songmei,ZHENG Chunying
E-mail:13039989361@163.com;zhengchunying68@163.com
CLC Number:
ZHANG He, YOU Mengyao, WAN Lu, YAN Jiajia, LIU Songmei, ZHENG Chunying. Identification and Fermentation Conditions of Syringin-producing Endophytic Fungus CJ7[J]. Chinese Agricultural Science Bulletin, 2022, 38(25): 143-150.
Add to citation manager EndNote|Ris|BibTeX
URL: https://www.casb.org.cn/EN/10.11924/j.issn.1000-6850.casb2021-0888
样品 | 大肠杆菌 | 枯草芽孢杆菌 | 金黄色葡萄球菌 | 短小芽孢杆菌 | 单增李斯特菌 | 鲍曼不动杆菌 | 肺炎克雷伯菌 | 铜绿假单孢菌 | 屎肠球菌 | 白假丝酵母 |
---|---|---|---|---|---|---|---|---|---|---|
CJ7 | 15.02±0.32 | 14.79±0.80 | 18.05±0.17 | 14.93±0.13 | 14.02±0.14 | 18.55±0.09 | 15.91±0.18 | 16.53±0.28 | 17.10±0.24 | 12.57±0.19 |
甲醇 | - | - | - | - | - | - | - | - | - | - |
链霉素 | 26.71±0.31 | 27.14±0.30 | 28.52±0.07 | 19.13±0.21 | 24.20±0.35 | 28.07±0.05 | 26.50±0.01 | 28.72±0.30 | 25.00±0.08 | - |
制霉素 | - | - | - | - | - | - | - | - | - | 19.90±0.05 |
样品 | 大肠杆菌 | 枯草芽孢杆菌 | 金黄色葡萄球菌 | 短小芽孢杆菌 | 单增李斯特菌 | 鲍曼不动杆菌 | 肺炎克雷伯菌 | 铜绿假单孢菌 | 屎肠球菌 | 白假丝酵母 |
---|---|---|---|---|---|---|---|---|---|---|
CJ7 | 15.02±0.32 | 14.79±0.80 | 18.05±0.17 | 14.93±0.13 | 14.02±0.14 | 18.55±0.09 | 15.91±0.18 | 16.53±0.28 | 17.10±0.24 | 12.57±0.19 |
甲醇 | - | - | - | - | - | - | - | - | - | - |
链霉素 | 26.71±0.31 | 27.14±0.30 | 28.52±0.07 | 19.13±0.21 | 24.20±0.35 | 28.07±0.05 | 26.50±0.01 | 28.72±0.30 | 25.00±0.08 | - |
制霉素 | - | - | - | - | - | - | - | - | - | 19.90±0.05 |
组别 | 因素 | 抑菌圈直径/mm | |||
---|---|---|---|---|---|
A | B | C | D | ||
1 | 1 | 1 | 1 | 1 | 25.83±0.30 |
2 | 1 | 2 | 2 | 2 | 28.33±0.30 |
3 | 1 | 3 | 3 | 3 | 29.33±0.30 |
4 | 2 | 1 | 2 | 3 | 30.50±0.00 |
5 | 2 | 2 | 3 | 1 | 29.17±0.70 |
6 | 2 | 3 | 1 | 2 | 30.67±0.70 |
7 | 3 | 1 | 3 | 2 | 23.50±0.00 |
8 | 3 | 2 | 1 | 3 | 21.67±0.40 |
9 | 3 | 3 | 2 | 1 | 26.67±0.20 |
K1 | 83.49 | 81.83 | 80.17 | 81.67 | ΣY=249.67 CT= 6926.12 |
K2 | 94.34 | 79.17 | 87.50 | 84.50 | |
K3 | 71.84 | 88.67 | 82.00 | 83.50 | |
R | 7.50 | 3.17 | 2.45 | 0.95 | |
S | 84.41 | 16.02 | 9.71 | 1.38 |
组别 | 因素 | 抑菌圈直径/mm | |||
---|---|---|---|---|---|
A | B | C | D | ||
1 | 1 | 1 | 1 | 1 | 25.83±0.30 |
2 | 1 | 2 | 2 | 2 | 28.33±0.30 |
3 | 1 | 3 | 3 | 3 | 29.33±0.30 |
4 | 2 | 1 | 2 | 3 | 30.50±0.00 |
5 | 2 | 2 | 3 | 1 | 29.17±0.70 |
6 | 2 | 3 | 1 | 2 | 30.67±0.70 |
7 | 3 | 1 | 3 | 2 | 23.50±0.00 |
8 | 3 | 2 | 1 | 3 | 21.67±0.40 |
9 | 3 | 3 | 2 | 1 | 26.67±0.20 |
K1 | 83.49 | 81.83 | 80.17 | 81.67 | ΣY=249.67 CT= 6926.12 |
K2 | 94.34 | 79.17 | 87.50 | 84.50 | |
K3 | 71.84 | 88.67 | 82.00 | 83.50 | |
R | 7.50 | 3.17 | 2.45 | 0.95 | |
S | 84.41 | 16.02 | 9.71 | 1.38 |
[1] | 国家药典委员会. 中华人民共和国药典[S]. 北京: 中国医药科技出版社, 2020:207. |
[2] | BAYGILDIEVA D I, BRAUN A V, STAVRIANIDI A N, et al. Determination of eleutheroside B and eleutheroside E in extracts from Eleutherococcus senticosus by liquid chromatography/mass spectrometry[J]. Journal of analytical chemistry, 2020, 75(14):1832-1837. |
[3] | SHI X L, YANG Y, REN H X, et al. Identification of multiple components in deep eutectic solvent extract of Acanthopanax senticosus root by ultra-high-performance liquid chromatography with quadrupole orbitrap masss pectrometry[J]. Phytochemistry letters, 2020, 35:175-185. |
[4] | LI X H, ZHANG Z Q, GUO Z H, et al. Acanthopanax senticosus polysaccharide-loaded calcium carbonate nanoparticle as an adjuvant to enhance porcine parvovirus vaccine immune responses[J]. Medicine in drug discovery, 2021, 11:1-10. |
[5] | JIA A L, ZHANG Y H, GAO H, et al. A review of Acanthopanax senticosus (Rupr and Maxim.) harms: From ethnopharmacological use to modern application[J]. Journal of ethnopharmacology, 2021, 286:1-17. |
[6] | HUANG L Z, HUANG B, YE Q, et al. Bioactivity-guided fractionation for anti-fatigue property of Acanthopanax senticosus[J]. Journal of ethnopharmacology, 2011, 133:213-219. |
[7] | ZHANG M L, SUN Y P, LIU Y, et al. Five new sesquiterpenoids from the fruits of Acanthopanax senticosus (Rupr. & Maxim.) Harms[J]. Fitoterapia, 2021, 149:1-6. |
[8] | XIA Y G, HUANG Y X, LIANG J, et al. Comparable studies of two polysaccharides from leaves of Acanthopanax senticosus: Structure and antioxidation[J]. International journal of biological macromolecules, 2020, 147:350-362. |
[9] | CHEN R H, DU W D, WANG Q, et al. Effects of Acanthopanax senticosus (Rupr. & Maxim.) Harms on cerebral ischemia-reperfusion injury revealed by metabolomics and transcriptomics[J]. Journal of ethnopharmacology, 2021, 264:1-12. |
[10] | ZHANG X Q, LAI Y C, WANG L, et al. Phenylpropanoid constituents from Acanthopanax senticosus[J]. Biochemical systematics and ecology, 2011, 39:861-863. |
[11] | LI N, SUN Y N, ZHANG L, et al. NF-kB inhibitory activities of triterpenoid glycosides from the stems of Acanthopanax senticosus (Rupr. & Maxim.) Harms[J]. Phytochemistry letters, 2016, 15:210-214. |
[12] | ZHAO C J, CHENG J B, LI C Y, et al. Quality evaluation of Acanthopanax senticosus via quantitative analysis of multiple components by single marker and multivariate data analysis[J]. Journal of pharmaceutical and biomedical analysis, 2021, 201:1-6. |
[13] | SUN H, HAN Y, ZHANG A, et al. UPLC-MS based metabolic profiling of the phenotypes of Acanthopanax senticosus reveals the changes in active metabolites distinguishing the diversities of the plant grown in northeast area of China[J]. Chinese journal of natural medicines, 2012, 10(3):196-206. |
[14] | BRADER G, COMPANT S, MITTER B, et al. Metabolic potential of endophytic bacteria[J]. Current opinion in biotechnology, 2014, 27:30-37. |
[15] | KAMBIZ B, NADER H, BEHROUZ H, et al. Isolation and identification of endophytic bacteria from potato tissues and their effects as biological control agents against bacterial wilt[J]. Physiological and molecular plant pathology, 2021, 116:1-9. |
[16] | 张萍, 吴丹, 矫佳陈琦, 等. 产原儿茶酸北五味子内生细菌WF17的鉴定及抑菌活性研究[J]. 中国食品学报, 2015, 15(7):198-202. |
[17] | LIU Y T, YOU Y X, LI Y W, et al. Characterization of carboxymethylated polysaccharides from Catathelasma ventricosum and their antioxidant and antibacterial activities[J]. Journal of functional foods, 2017, 38:355-362. |
[18] | 邵力平, 沈瑞祥, 张素轩, 等. 真菌分类学[M]. 北京: 中国林业出版社, 1984:17. |
[19] | 魏景超. 真菌鉴定手册[M]. 上海: 上海科学技术出版社, 1982:84. |
[20] | KATHAWUT S, WANPEN L, ANYALAK W, et al. Biological potential and chemical composition of bioactive compounds from endophytic fungi associated with thai mangrove plants[J]. South African journal of botany, 2021, 141:66-76. |
[21] | YAN J H, TONG S Q, CHU J J, et al. Preparative isolation and purification of syringin and edgeworoside C from Edgeworthia chrysantha Lindl by high-speed counter-current chromatography[J]. Journal of chromatography A, 2004, 1043:329-332. |
[22] | LEE C H, HUANG C W, CHANG P C, et al. Reactive oxygen species mediate the chemopreventive effects of syringin inbreast cancer cells[J]. Phytomedicine, 2019, 61:1-12. |
[23] | TAN J Y, LUO J, MENG C C, et al. Syringin exerts neuroprotective effects in a rat model of cerebral ischemia through the FOXO3a/NF-κB pathway[J]. International immunopharmacology, 2021, 90:1-9. |
[24] | 郭晓璐, 卢玢宇, 郑春英. 刺五加内生细菌的分离及其抑菌活性研究[J]. 中国新药杂志, 2014, 23(22):128-135. |
[25] | 熊亚南, 邢朝斌, 吴鹏, 等. 刺五加内生真菌分离及分布研究[J]. 安徽农业科学, 2009, 37(24):11347-11348. |
[26] | 徐红艳, 刘爱华, 王彬, 等. 刺五加内生放线菌的分离与多样性研究[J]. 西南师范大学学报:自然科学版, 2016, 41(5):28-35. |
[27] | 郑春英, 崔宇, 纪鑫, 等. 药用植物刺五加内生真菌的分离及其抑菌活性研究[J]. 中国药学杂志, 2008, 43(20):1541-1544. |
[28] | 郑帅. 刺五加内生真菌抗氧化活性研究[D]. 哈尔滨: 黑龙江中医药大学, 2015. |
[29] | 邢朝斌, 熊亚南, 劳凤云, 等. 刺五加内生真菌对刺五加苷B和E含量的影响[J]. 安徽农业科学, 2009, 37(25):12010-12011. |
[1] | GONG Yongyong, DUANMU Huizi. TIFY Gene Family in Sugar Beet: Whole Genome Identification and Bioinformatics Analysis [J]. Chinese Agricultural Science Bulletin, 2022, 38(8): 17-24. |
[2] | WU Jiajia, ZHU Jiahong, CAO Aocheng, YAN Dongdong, WANG Qiuxia, ZHANG Chunhua, LI Yuan. Amomum tsao-ko in Yunnan Province: Identification of Major Pathogenic Fungi and Screening of Control Fungicides [J]. Chinese Agricultural Science Bulletin, 2022, 38(6): 121-128. |
[3] | SHANG Na, REN Aizhi, LIU Bing, ZHAO Peibao. Isolation and Identification of the Pathogen Causing Leaf Spot on Trifolium repens [J]. Chinese Agricultural Science Bulletin, 2022, 38(4): 81-85. |
[4] | WU Fang, FENG Liguo, JIANG Xingjian, SHI Yuelong, WANG Chunhui, HUANG Xiaohui, YU Chuquan. Habitat Investigation and Identification of Three Wild Edible Fungi in Dongting Lake Area [J]. Chinese Agricultural Science Bulletin, 2022, 38(34): 27-32. |
[5] | YANG Yiwei, LIU Chen, WEI Peiyao, LI Yingmei. Current Situation and Virus Identification of Sweet Potato Virus Disease (SPVD) in Shaanxi Province [J]. Chinese Agricultural Science Bulletin, 2022, 38(33): 109-115. |
[6] | WEI Rifeng, PENG Chengbin, CHEN Meixia, ZHANG Zhengxiong, RUAN Junfeng, LIU Wei. The Pathogen Identification and Its Biological Characteristics of Chimonanthus salicifolius Leaf Spot Disease [J]. Chinese Agricultural Science Bulletin, 2022, 38(3): 127-133. |
[7] | LAI Jia, WEI Shugu, HUANG Ling, SHENG Yuzhen, LIU Yong, ZHANG Qianfang, YE Pengsheng. Identification and Evaluation on Bolting Traits of Chinese Cabbage Group Germplasm Resources [J]. Chinese Agricultural Science Bulletin, 2022, 38(28): 41-47. |
[8] | YOU Mengyao, WAN Lu, YAN Jiajia, ZHANG He, ZHENG Chunying. Research Progress on Endophytes from Glycyrrhiza uralensis [J]. Chinese Agricultural Science Bulletin, 2022, 38(26): 20-26. |
[9] | LIU Danyang, CUI Rufei, GENG Gui, WANG Yuguang. Pathogenic Bacteria of Sugar Beet Blight: Isolation and Identification [J]. Chinese Agricultural Science Bulletin, 2022, 38(24): 113-117. |
[10] | ZHEN Jincheng, MU Yuting, SI Lu, YU Hongjia, DU Tingting, SHAN Tijiang, XU Lijian. Studies on the Antimicrobial Activity of Litter Fungus Berkleasmium sp. and Its Spirobisnaphthalenes [J]. Chinese Agricultural Science Bulletin, 2022, 38(22): 115-120. |
[11] | LI Wangsheng, WANG Xueqian, YIN Xilong, SHI Yang, XING Wang. Drought Resistance of Sugar Beet Seedling: Identification and Index Screening [J]. Chinese Agricultural Science Bulletin, 2022, 38(21): 17-23. |
[12] | ZHANG Fuli, ZHANG Yirong, LAN Qingkuo, YANG Xiaofeng, LIU Wei, FU Shaobing, LIU Qian, CHEN Min, YIN Quan. A Specific PCR Method for Identifying the Authentic Medicinal Material Bulbus Fritillariae cirrhosae [J]. Chinese Agricultural Science Bulletin, 2022, 38(19): 59-65. |
[13] | QIU Tianyi, XU Yue, ZHEN Jincheng, SI Lu, YU Hongjia, MU Yuting, XU Lijian. Active Fungi from Forest Litter of the Greater Khingan Mountains and Their Metabolites [J]. Chinese Agricultural Science Bulletin, 2022, 38(18): 122-127. |
[14] | LI Junping, WANG Xiuping, LIU Sujuan, LU Xuelin, WU Zhe. Salt Tolerance of Maize at Seedling Stage: Identification and Evaluation Method [J]. Chinese Agricultural Science Bulletin, 2022, 38(18): 28-34. |
[15] | CHEN Yunkun, HU Chunyan, ZHANG Zhiyu, ZHAO Yanfang, CAO Hui. Antimicrobial Activity of Extracts from Five Thymelaeaceae Plants Against Seven Plant Pathogenic Fungi [J]. Chinese Agricultural Science Bulletin, 2022, 38(13): 148-156. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||