Effect of Different Superovulation Schedules and Donor Cows Ages on Production Efficiency of Sexed Embryos for High Yielding Holstein Cows in Vitro

doi:10.11924/j.issn.1000-6850.casb2022-0907

Abstract

Abstract:

The purpose of the study is to investigate the effects of hormone treatment and donor age on superovulation and production efficiency of sex control embryos for high yielding Holstein cows in vitro, and provide technical support for the establishment of stable and efficient superovulation schedule and the improvement of production efficiency of sex control embryos for high yielding Holstein cows in vitro. A total of 60 high yielding Holstein cows were randomly selected and divided into 3 groups. OPU-IVF for embryo production was performed according to FSH injection schedule: bi-weekly (BW), once weekly (OW) and no injection (CON) in vitro. The best schedule was used to further study whether there was difference in embryo production efficiency in vitro between young cattle and postpartum cattle. The superovulation effect of BW group was better than that of OW and CON groups. The number of available ovum (15.30±0.60), the proportion of available ovum (68.83%±1.92%), the number of grade A ovum (9.86±0.38) and the number of grade B ovum (5.44±0.37) in BW group were significantly higher than those of OW group. The in vitro development of IVF early embryos in BW group was better than that in OW and CON groups. The cleavage rate (77.80%±1.08%) and the number of embryos per cow (5.11±0.27) in BW group were higher than those of OW and CON groups, and the differences were significant. The proportion of grade B embryos (13.95%±1.23%) in BW and CON groups was significantly lower than that in OW group. The number of average ovum (27.10±1.35) and embryo per cow (5.29±0.28) in postpartum cattle group were significantly higher than those of young cattle group, but the ovum availability was significantly lower than that of young cattle group, which was 64.44%±1.81% and 75.01%±2.33%, respectively. The BW group had the best superovulation schedule, under which the production efficiency of sex control embryos in vitro of postpartum cattle was significantly higher than that of young cattle.

Key words: high yielding Holstein cows, FSH, superovulation, ovum pick-up, sex control embryos production in vitro

SUN Wei, LV He, WANG Chao, ZHAO Yongchao, GUO Yaxin, WANG Feifei, LIU Tao, WANG Maojun, BAO Xiangnan, ZHANG Bozhao, Cao Guifang, LI Haijun, LI Xihe. Effect of Different Superovulation Schedules and Donor Cows Ages on Production Efficiency of Sexed Embryos for High Yielding Holstein Cows in Vitro[J]. Chinese Agricultural Science Bulletin, 2023, 39(10): 130-135.

Figures/Tables 4

References 26

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方案	时间	D1	D3(FSH)	D4(FSH)	D6	D8	D15
方案一(BW)	08:00	去优势卵泡	100 IU	100 IU	OPU采卵	—	去优势卵泡（重复D1-D14）
方案一(BW)	18:00	去优势卵泡	100 IU	100 IU	OPU采卵	—	去优势卵泡（重复D1-D14）
方案二(OW)	08:00	去优势卵泡	80 IU	60 IU	OPU采卵	去优势卵泡（重复D1-D6）	—
方案二(OW)	18:00	去优势卵泡	60 IU	40 IU	OPU采卵	去优势卵泡（重复D1-D6）	—
方案三(CON)	08:00	OPU	—	—	OPU	—	—

方案	时间	D1	D3(FSH)	D4(FSH)	D6	D8	D15
方案一(BW)	08:00	去优势卵泡	100 IU	100 IU	OPU采卵	—	去优势卵泡（重复D1-D14）
方案一(BW)	18:00	去优势卵泡	100 IU	100 IU	OPU采卵	—	去优势卵泡（重复D1-D14）
方案二(OW)	08:00	去优势卵泡	80 IU	60 IU	OPU采卵	去优势卵泡（重复D1-D6）	—
方案二(OW)	18:00	去优势卵泡	60 IU	40 IU	OPU采卵	去优势卵泡（重复D1-D6）	—
方案三(CON)	08:00	OPU	—	—	OPU	—	—

方案	采卵头次/头	头均卵子总数/枚	头均可用卵数/枚	可用卵占比/%	头均A级卵数/枚	头均B级卵数/枚
FSH两周采卵一次(BW)	80	22.37±1.16a	15.30±0.60a	68.83±1.92a	9.86±0.38a	5.44±0.37a
FSH一周采卵一次(OW)	100	19.60±0.85a	11.61±0.53b	59.40±1.81b	7.33±0.61b	4.29±0.41b
FSH未注射(CON)	120	13.60±0.82b	9.05±0.63c	66.38±0.94a	5.49±0.49c	3.56±0.18b

方案	采卵头次/头	头均卵子总数/枚	头均可用卵数/枚	可用卵占比/%	头均A级卵数/枚	头均B级卵数/枚
FSH两周采卵一次(BW)	80	22.37±1.16a	15.30±0.60a	68.83±1.92a	9.86±0.38a	5.44±0.37a
FSH一周采卵一次(OW)	100	19.60±0.85a	11.61±0.53b	59.40±1.81b	7.33±0.61b	4.29±0.41b
FSH未注射(CON)	120	13.60±0.82b	9.05±0.63c	66.38±0.94a	5.49±0.49c	3.56±0.18b

方案	采卵头次/头	卵裂率/%	头均胚胎数/枚	A级胚胎占比/%	B级胚胎占比/%	囊胚率/%
FSH两周采卵一次(BW)	80	77.80±1.08a	5.11±0.27a	82.02±1.96a	13.95±1.23a	33.42±1.02a
FSH一周采卵一次(OW)	100	70.33±1.43b	3.00±0.03b	77.47±1.30a	19.60±1.21b	26.17±1.09b
FSH未注射(CON)	120	70.88±1.76b	2.58±0.28b	79.25±2.53a	15.14±1.21a	28.09±1.05b