Abstract:

The purpose of this experiment is to study the effects of different concentrations of pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotrophin (HCG) on superovulation in rabbits. Twenty-four five-month-old pubertal rabbits were randomly divided into four groups, one of which was the control group, the other three groups were injected with 50 IU, 70 IU and 90 IU PMSG respectively and 100 IU HCG. After the injection, the rabbits were mated, and dissected and embryos removed 24 hours later. The physical state of fallopian tube and the quality and quantity of embryos were observed and analyzed. The results showed that with the increase of PMSG injection concentration, the change of fallopian tube was not significant; the number of congested follicles and abnormal embryos increased and the difference among the groups was significant (P<0.01), while the number of high-quality embryos increased first and then decreased. When PMSG+HCG injection was 70 IU+100 IU, 213 high-quality embryos were obtained at most. The combination of superovulation at different concentrations of PMSG+HCG did not affect the physical fallopian tube morphology in rabbits, but the effect of superovulation was different. The rate of embryonic malformation increased with the increase of PMSG concentration. Therefore, it is recommended that the concentration of PMSG +HCG should be 70 IU+100 IU in practice.

Key words: PMSG, HCG, rabbits, superovulation