Analysis of Soil Fungal Diversity in Zhaotong Core Tobacco-growing Area Based on High-throughput Sequencing

doi:10.11924/j.issn.1000-6850.casb2023-0087

Abstract

Abstract:

High-throughput sequencing technology was used to explore the soil fungal diversity from the molecular level in new tobacco area, continuous cropping area with serious soil-borne diseases and typical rotation area in eight tobacco-growing districts in Zhaotong. The results showed that the soil fungal community included 30 phyla, 79 classes, 202 orders, 443 families and 976 genera, and Ascomycota was the absolute dominant phylum, Mortierella and Penicillium were the dominant genera of the soil fungal community. The analysis of community indicator species showed that the relative abundance of the soil fungal community of Ascochyta in new tobacco area was significantly higher than that in continuous cropping area and rotation area. The relative abundance of Purpureocillium, Pseudogymnoascus and Talaromyces in continuous cropping area were significantly higher than that in new tobacco area and rotation area, Fusarium in rotation area was significantly higher than that in new tobacco area, and Mortierella in rotation area was significantly higher than that in continuous cropping area. From the perspective of biodiversity characteristics, soil fungal community in new tobacco area had the highest value, and the species richness was significantly higher than that in continuous cropping area and rotation area. The species richness of soil fungal community in rotation area was significantly higher than that in continuous cropping area, the species evenness was significantly lower than that in continuous cropping area, and the biodiversity of fungal community was slightly higher than that in continuous cropping area. From the perspective of the function of fungal community, the pathotroph, saprotroph, and saprotroph-symbiotroph were main components. In new tobacco area, the top 1 to top 3 of the fungal community were saprotroph-symbiotroph, saprotroph and Pathotroph, that of the continuous cropping area were saprotroph, pathotroph and saprotroph-symbiotroph, and that of the rotation area were saprotroph-symbiotroph, pathotroph-saprotroph-symbiotroph, and saprotroph. The composition of fungal community in tobacco planting soil in Zhaotong was rich. The biodiversity of soil fungal community in new tobacco planting area was the highest, and the species richness was significantly higher than that in continuous cropping area and rotation area. There was no significant difference in the composition of fungal community structure among different types of soil. Rotation was beneficial to reducing the pathotrophic fungal community and the probability of fungal diseases.

Key words: high-throughput sequencing, tobacco-planting soil, community composition, fungal diversity

NI Xia, GONG Lin, CHEN Hua, SONG Mingjian, ZHOU Yuzhong, CHEN Min, FU Yeming, GONG Haohan, PAN Wenzheng, ZHANG Mengsheng, LUO Qipeng, LI Jianyun, BAO Hongming, LI Dewen, ZHANG Haikun. Analysis of Soil Fungal Diversity in Zhaotong Core Tobacco-growing Area Based on High-throughput Sequencing[J]. Chinese Agricultural Science Bulletin, 2024, 40(2): 61-70.

Figures/Tables 17

References 28

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类型	测序区域	引物名称	引物序列	产物长度
ITS	ITS2	ITS3_KYO2	GATGAAGAACGYAGYRAA	381
ITS	ITS2	ITS4	TCCTCCGCTTATTGATATGC	381

类型	测序区域	引物名称	引物序列	产物长度
ITS	ITS2	ITS3_KYO2	GATGAAGAACGYAGYRAA	381
ITS	ITS2	ITS4	TCCTCCGCTTATTGATATGC	381

门	新烟区(A)	连作区(B)	轮作区(E)
子囊菌门Ascomycota	75.38	81.36	73.95
被孢霉门Mortierellomycota	14.06	9.48	16.05
担子菌门Basidiomycota	7.72	6.68	6.95
壶菌门Chytridiomycota	0.48	0.77	0.86
毛霉门Mucoromycota	0.24	0.45	0.23
球囊菌门Glomeromycota	0.11	0.06	0.09
其他	0.11	0.03	0.04
未归类	1.90	1.16	1.84

门	新烟区(A)	连作区(B)	轮作区(E)
子囊菌门Ascomycota	75.38	81.36	73.95
被孢霉门Mortierellomycota	14.06	9.48	16.05
担子菌门Basidiomycota	7.72	6.68	6.95
壶菌门Chytridiomycota	0.48	0.77	0.86
毛霉门Mucoromycota	0.24	0.45	0.23
球囊菌门Glomeromycota	0.11	0.06	0.09
其他	0.11	0.03	0.04
未归类	1.90	1.16	1.84

属	新烟区(A)	连作区(B)	轮作区(E)
被孢霉属Mortierella	14.06	9.48	16.05
青霉菌属Penicillium	9.13	9.21	7.98
拟青霉菌属Purpureocillium	1.55	6.72	1.75
曲霉菌属Aspergillus	1.80	3.80	4.00
假裸囊菌属Pseudogymnoascus	2.55	4.11	1.33
镶刀菌属Fusarium	1.75	2.76	3.65
腐质霉属Humicola	2.56	1.89	2.22
壳二胞菌属Ascochyta	2.55	1.36	1.29
篮状菌属Talaromyces	1.65	1.89	0.81
柱孢属Cylindrocarpon	0.51	1.47	2.15
其他	28.93	27.00	29.01
未归类	32.97	30.33	29.78