The Inoculation Effect of Mycorrhizal Fungi in Tissue Culture Seedlings of Anoectochilus roxburghii (Wall.) Lindl. and Bletilla striata (Thunb. ex A. Murray) Rchb. f.

doi:10.11924/j.issn.1000-6850.casb2023-0506

Abstract

Abstract:

This study was conducted to further explore the mutual relationship between tissue-cultured seedlings of Anoectochilus roxburghii (Wall.) Lindl. and Bletilla striata (Thunb. ex A. Murray) Rchb. f. and inoculated strains in the arbuscular mycorrhizal cultivation system. Tissue-cultured seedlings of Anoectochilus roxburghii (Wall.) Lindl. and Bletilla striata (Thunb. ex A. Murray) Rchb. f. were used as materials, and the arbuscular mycorrhizal cultivation system was established by inoculating strains. The morphological and physiological indicators of the tissue-cultured seedlings at different growth stages were measured to compare their responses to different strains. The A2 strain of Anoectochilus roxburghii (Wall.) Lindl. had a significant promoting effect on the aboveground and underground growth of tissue-cultured seedlings. The average fresh weight growth rate of A2 treatment was 20.9% and 19.1% higher than that of A1 and CK1 treatments. The number of leaves, plant height, root growth rate, number of roots, and root length of A2 treatment were 21.7%, 2.7 cm, 16.9%, 48.3%, and 65.8% higher than those of CK1 treatment, respectively. The B1 and B2 strains of Bletilla striata (Thunb. ex A. Murray) Rchb. f. promoted the aboveground and underground growth of tissue-cultured seedlings. The growth indicators of B1 and B2 treatments were higher than the control. The fresh weight growth rate, number of leaves, plant height, root growth rate, number of roots, and root length of B1 treatment were 3.9%, 41.8%, 3.7 cm, 25.8%, 33.0%, and 52.6% higher than those of CK2 treatment, respectively. In conclusion, except for the negative impact of the A1 strain in the early stage, other strains had a positive promoting effect on the growth of tissue-cultured seedlings throughout the symbiotic cultivation period.

Key words: Anoectochilus roxburghii (Wall.) Lindl., Bletilla striata (Thunb. ex A. Murray) Rchb. f., tissue-cultured seedlings, mycorrhizal fungi, inoculation, symbiotic culture, chlorophyll content, morphological index

LI Jinghong, ZHANG Lihua, ZHONG Jing. The Inoculation Effect of Mycorrhizal Fungi in Tissue Culture Seedlings of Anoectochilus roxburghii (Wall.) Lindl. and Bletilla striata (Thunb. ex A. Murray) Rchb. f.[J]. Chinese Agricultural Science Bulletin, 2024, 40(24): 122-128.

Figures/Tables 5

References 31

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