Effect of Oligosaccharides Combined with Purpureocillium lilacinum on Efficacy of Chemical Nematicides on Flue-cured Tobacco Root-knot Nematode Disease

doi:10.11924/j.issn.1000-6850.casb2023-0834

Abstract

Abstract:

In order to better control the harm of tobacco root-knot nematode and reduce the dosage of chemical nematocides, oligosaccharide immunoinducer was used together with fosthiazate, avermectin and Purpureocillium lilacinum in field plot experiment to study the control effect on tobacco root-knot nematode (RKN). The results in 2021 showed that the synergistic application of 15 L/hm² chitosan oligosaccharides combined with 22.5 kg/hm²fosthiazate, or 15 L/hm² chitin oligosaccharides combined with 22.5 kg/hm² fosthiazate had a significantly higher control effect to RKN than that of fosthiazate alone. The relative control effect of chitosan oligosaccharides combined with fosthiazate, chitin oligosaccharides combined with fosthiazate treatment was 64.42% and 65.38%, respectively, and that of fosthiazate treatment was 45.20%. These two treatments significantly promoted the growth of flue-cured tobacco. The results in 2022 showed that when 1 kg/hm² chitin oligosaccharides, 15 kg/hm² P. lilacinum and 16.875 kg/hm²fosthiazate (25% reduction) were used together, the relative control effect to RKN and overall effect on tobacco growth was better than that of normal amount (22.5 kg/hm²) and 50% reduction of fosthiazate (11.25 kg/hm²). Therefore, the synergistic application of oligosaccharide with fosthiazate, and oligosaccharide with P. lilacinum and fosthiazate can improve the control effect of tobacco root-knot nematode disease and reduce the amount of chemical nematicides, and which can be used as one of the effective control technologies for plant nematode disease control.

Key words: tobacco root-knot nematodes disease, oligosaccharide plant immune inducer, Purpureocillium lilacinum, fosthiazate, control effect, integrated pest management, pesticide reduction

ZHAO Xiaoming, ZHANG Xuehui, DAI Kuai, LI Jiangzhou, CAO Hailong, XU Shuang, ZHANG Cuiping, XUE Meng, CUI Yonghe, YIN Heng. Effect of Oligosaccharides Combined with Purpureocillium lilacinum on Efficacy of Chemical Nematicides on Flue-cured Tobacco Root-knot Nematode Disease[J]. Chinese Agricultural Science Bulletin, 2024, 40(24): 133-142.

Figures/Tables 11

References 31

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编号	处理	移栽时	移栽后40 d
处理1	壳寡糖+阿维菌素	阿维菌素45 kg/hm²，穴施；壳寡糖制剂15 L/hm²，稀释1000倍灌根	阿维菌素45 kg/hm²，环施；壳寡糖制剂15 L/hm²，稀释1000倍灌根
处理2	几丁寡糖+阿维菌素	阿维菌素45 kg/hm²，穴施；几丁寡糖制剂15 L/hm²，稀释1000倍灌根	阿维菌素45 kg/hm²，环施；几丁寡糖制剂15 L/hm²，稀释1000倍灌根
处理3	壳寡糖+噻唑膦	10%噻唑膦22.5 kg/hm²，穴施；壳寡糖制剂15 L/hm²，稀释1000倍灌根	10%噻唑膦22.5 kg/hm²，环施；壳寡糖制剂15 L/hm²，稀释1000倍灌根
处理4	几丁寡糖+噻唑膦	10%噻唑膦22.5 kg/hm²，穴施；几丁寡糖制剂15 L/hm²，稀释1000倍灌根	10%噻唑膦22.5 kg/hm²，环施；几丁寡糖制剂15 L/hm²，稀释1000倍灌根
处理5	阿维菌素	阿维菌素45 kg/hm²，穴施，灌清水15 t/hm²	阿维菌素45 kg/hm²，环施，灌清水15 t/hm²
处理6	噻唑膦	10%噻唑膦22.5 kg/hm²，穴施，灌清水15 t/hm²	10%噻唑膦22.5 kg/hm²，环施，灌清水15 t/hm²
处理7	CK	灌清水15 t/hm²	灌清水15 t/hm²

编号	处理	移栽时	移栽后40 d
处理1	壳寡糖+阿维菌素	阿维菌素45 kg/hm²，穴施；壳寡糖制剂15 L/hm²，稀释1000倍灌根	阿维菌素45 kg/hm²，环施；壳寡糖制剂15 L/hm²，稀释1000倍灌根
处理2	几丁寡糖+阿维菌素	阿维菌素45 kg/hm²，穴施；几丁寡糖制剂15 L/hm²，稀释1000倍灌根	阿维菌素45 kg/hm²，环施；几丁寡糖制剂15 L/hm²，稀释1000倍灌根
处理3	壳寡糖+噻唑膦	10%噻唑膦22.5 kg/hm²，穴施；壳寡糖制剂15 L/hm²，稀释1000倍灌根	10%噻唑膦22.5 kg/hm²，环施；壳寡糖制剂15 L/hm²，稀释1000倍灌根
处理4	几丁寡糖+噻唑膦	10%噻唑膦22.5 kg/hm²，穴施；几丁寡糖制剂15 L/hm²，稀释1000倍灌根	10%噻唑膦22.5 kg/hm²，环施；几丁寡糖制剂15 L/hm²，稀释1000倍灌根
处理5	阿维菌素	阿维菌素45 kg/hm²，穴施，灌清水15 t/hm²	阿维菌素45 kg/hm²，环施，灌清水15 t/hm²
处理6	噻唑膦	10%噻唑膦22.5 kg/hm²，穴施，灌清水15 t/hm²	10%噻唑膦22.5 kg/hm²，环施，灌清水15 t/hm²
处理7	CK	灌清水15 t/hm²	灌清水15 t/hm²

编号	处理	移栽时	移栽后40 d
处理1	几丁寡糖+噻唑膦	几丁寡糖粉剂750 g/hm²， 10%噻唑膦22.5 kg/hm²，穴施	几丁寡糖制剂15 L/hm²，噻唑膦微囊悬浮剂7.5 L/hm²，灌根
处理2	几丁寡糖+噻唑膦减量25%	几丁寡糖粉剂750 g/hm²， 10%噻唑膦16.875 kg/hm²，穴施	几丁寡糖制剂15 L/hm²，唑磷微囊悬浮剂5.625 L/hm²，灌根
处理3	几丁寡糖+菌剂+噻唑膦	几丁寡糖粉剂750 g/hm²，10%噻唑膦22.5 kg/hm²，菌剂15 kg/hm²，穴施	几丁寡糖制剂15 L/hm²，噻唑膦微囊悬浮剂7.5 L/hm²，灌根
处理4	几丁寡糖+菌剂+噻唑膦减量25%	几丁寡糖粉剂750 g/hm²，10%噻唑膦16.875 kg/hm²，菌剂15 kg/hm²，穴施	几丁寡糖制剂15 L/hm²，唑磷微囊悬浮剂5.625 L/hm²，灌根
处理5	几丁寡糖+菌剂+噻唑膦（减量50%）	几丁寡糖粉剂750 g/hm²，10%噻唑膦11.25 kg/hm²，菌剂15 kg/hm²，穴施	几丁寡糖制剂15 L/hm²，唑磷微囊悬浮剂3.75 L/hm²，灌根
处理6	CK	不施药	灌清水15 t/hm²

编号	处理	移栽时	移栽后40 d
处理1	几丁寡糖+噻唑膦	几丁寡糖粉剂750 g/hm²， 10%噻唑膦22.5 kg/hm²，穴施	几丁寡糖制剂15 L/hm²，噻唑膦微囊悬浮剂7.5 L/hm²，灌根
处理2	几丁寡糖+噻唑膦减量25%	几丁寡糖粉剂750 g/hm²， 10%噻唑膦16.875 kg/hm²，穴施	几丁寡糖制剂15 L/hm²，唑磷微囊悬浮剂5.625 L/hm²，灌根
处理3	几丁寡糖+菌剂+噻唑膦	几丁寡糖粉剂750 g/hm²，10%噻唑膦22.5 kg/hm²，菌剂15 kg/hm²，穴施	几丁寡糖制剂15 L/hm²，噻唑膦微囊悬浮剂7.5 L/hm²，灌根
处理4	几丁寡糖+菌剂+噻唑膦减量25%	几丁寡糖粉剂750 g/hm²，10%噻唑膦16.875 kg/hm²，菌剂15 kg/hm²，穴施	几丁寡糖制剂15 L/hm²，唑磷微囊悬浮剂5.625 L/hm²，灌根
处理5	几丁寡糖+菌剂+噻唑膦（减量50%）	几丁寡糖粉剂750 g/hm²，10%噻唑膦11.25 kg/hm²，菌剂15 kg/hm²，穴施	几丁寡糖制剂15 L/hm²，唑磷微囊悬浮剂3.75 L/hm²，灌根
处理6	CK	不施药	灌清水15 t/hm²

编号	株高/cm	叶片数	最大叶长/cm	最大叶宽/cm
处理1	29.37c	13.83ab	35.80b	21.35b
处理2	30.80c	13.54b	36.30b	22.23b
处理3	38.97a	14.67a	42.57a	25.00a
处理4	38.73a	14.80a	42.15a	25.58a
处理5	30.00c	13.63b	36.18b	21.73b
处理6	34.93b	14.17ab	38.83b	22.75b
处理7(CK)	21.43d	11.53c	28.93c	17.13c