Abstract: In order to give reason for generalizing high-protein and high-yield cultural integrative technology system of rice, two combinations of Peiliangyou288(high protein) and Peiliangyou210(low protein) were used to study the effects of high-protein and high-yield cultural integrative technology system of rice, san-zhuang-san-gao cultivation and practice cultivation on the activities of GOT and AK in functional leaves and grains of late cropping hybrid rice, based on the field experiment with the split-plot design.The results showed that high-protein and high-yield cultural integrative technology system of rice can obviously improve activities of GOT and AK in functional leaves and grains of Peiliangyou288 and Peiliangyou210, compared with san-zhuang-san-gao cultivation and practice cultivation. In functional leaves activities of GOT and AK on waxy stage increased by 11.41%~27.72% and 7.96%~47.45% respectively and activity of AK on maturing stage increased by 21.61%~163.76%, In grains activities of GOT and AK on maturing stage increased by 127.33%~175.36% and 16.36%~89.13% respectively.In addition, the contents of protein in brown rice increased by 1.61g/kg~23.26g/kg and the contents of amino acids in brown rice increased by 2.24%~44.09%. In these amino acids the contents of 7 kinds of amino acids essential for human health(Lys, Thr, Val, Met, Leu, Ile, Phe) in brown rice increased by 9.20%~58.05% and the contents of 11 kinds of amino acids essential for pig health(Lys, Thr, Val, Met, Leu，Ile，Phe, His, Arg, Cys, Tyr) in brown rice 4.47%~66.36%.