Abstract: In this paper, the distant hybridization of Natural Green Cotton GC1011(Gossypium hirsutum L.) and Seaisland cotton 324 (Gossypium barbadense L.) is reported. The character of each F1 plant is stable. Separation of F2 plants is crazy. Four stable hybrids, e.g G324-1, G324-6, 324-1 and 324-24 line were obtained by orthoselected for 5 years. The fibre is natural green. The lengths of fibre are 28.3mm, 27.8mm, 28.5mm, 28.1mm, and their total yields per ha are 4962kg, 4936.5 kg, 4929kg and 4956 kg. They have higher resistence to aphides and cotton-wilt. Therefore, it is suggest that they could be applicated in cultivating.