Abstract: The regeneration rate of adventitious buds from leaves plays an important role on the efficiency of gene transformation. In order to set up the stable and high efficient system of adventitious bud regeneration from leaves in vitro in grape, the basic medium component, the types and concentration of plant growth regulators, and the sources of carbon were studied on the regeneration by using the top three young leaves of seedless grape of Summer royal. The results showed that the adventitious buds could be induced by using the media of WPM, 1/2MS, B5, and NN69 with no significant difference. The applying of 10~20 g/L Glucose or commercial granulated sugar as the carbon source was adopted. The supply of 3.0 mg/L 6-BA and 0.05 mg/L IAA benefited the adventitious bud induction and the plantlet growth. The supply of TDZ was not recommended due to the more disorder buds induced and poor plantlet growth, even though it could obtain plenty adventitious buds.