Abstract: Ninety-six strains of Trichoderma were isolated from one hundred seventy samples of soil and other materials collected from fourteen regions in Ningxia. The morphological identification work indicated that they belonged to nine species of Trichoderma and two unidentified Trichoderma species，namely Trichoderma harzianum、T.viride、T.longibrachiatum、 T.koningii、T.pseudokoningii、T.fertile、T.aureoviride、T.atroviride、T.hamatum、T.sp1 and T.sp2. The results of competition and face-to-face-culturing showed that there were different antagonisms between eleven Trichoderma species and Botrytis cinerea，especially, inhibition of Trichoderma harzianum、T.viride、T.longibrachiatum and T.koningii were more obvious.Their inhibition index and inhibition rates repectively reachedⅠ~Ⅱand 61.8%~77.6%.By light microscopy,it was revealed that hyphae of Trichoderma species developed in coiled or penetrated Botrytis cinerea’s hyphae,and lead to its hyphae breaking or dissolving and cell cytoplasm concentration.