Abstract: The effect of inorganic salts on the production of endo-β-D-1,4-glucanase of cellulose-degrading fungi was investigated in this research. A orthogonal design was applied to design sixteen combinations which included four factors and two level, and the interaction was considered. Evaluating the impact significance of various components on the production of enzyme by the visual analysis and variance analysis with the index of enzymatic activity. The result showed that K2HPO4?3H2O had the positive remarkable effect on the production of enzme in the range of this research, but FeSO4?7H2O was the reverse. he optimized combination was as follows, 0.1g CaCI2, 0.1g FeSO4?7H2O, 0.5g K2HPO4?3H2O and 0.5g MgSO4?7H2O in every litre of liquid medium, the enzyme activity was improved 111.916% compared to the control without adding inorganic salts. The result of statistical analysis indicated that the low concentration of FeSO4?7H2O and the high concentration of K2HPO4?3H2O，CaCI2 and MgSO4?7H2O can achieve the maximum of enzyme production.