Current issue

• 2018 Vol.34 No.29 Published:15 October 2018

  The Amounts of Seed Sowing and Fertilizer Affect Yield and Nutrients Uptake of Ratooning Rice‘Xinliangyou 233’ 2018, 34 (29):  1-7.  DOI: 10.11924/j.issn.1000-6850.casb17070146 Abstract + ( 582 )   Aiming at providing the basis for the development of ratooning rice of our country, the effect of different number of seeds and different amount of fertilizer on the yield and nutrient uptake of plant about ‘Xinliangyou 233’ratooning rice cultivated on the yellow-brown soil in Hubei province were explored. The results were as follows: In both the main crop season and ratoon crop season, the yield of the Z1F2 model was the highest, which was 17.83 kg/plot and 11.36kg/plot, respectively. The amount of fertilizer had much more influence than the number of seeds and the medium-use (F2) fertilizer was the best. Both the amount of fertilizer and number of seeds affected the nutrient uptake of the plant, in a word , the absorption of N, P element had a mutual promotion effect but the absorption of K and N, P element had an opposite effect. According to the effect of different number of seeds and different amount of fertilizer on the yield and nutrient uptake of plant about‘Xinliangyou 233’ratooning rice, the recommended model is the Z1F2 model when cultivated on the yellow-brown soil.

  Nitrogen Rate and Transplanting Density: Effect on Yield Traits of Hybrid Rice‘Shuyou 217’ 2018, 34 (29):  8-12.  DOI: 10.11924/j.issn.1000-6850.casb18010148 Abstract + ( 786 )   The nitrogen fertilizer level and population density are two important factors that affect high yield of rice. To guide the production of‘Shuyou 217’, an experiment is conducted under the double cropping system of rice and wheat in Chengdu Plain in 2016 and 2017. The results showed that the yield reached 10.92 t/hm2 of A4B2 (nitrogen fertilizer 180 kg/hm2, planting density 14.31 hill/m2), increased by 1.19 t/hm2 compared with that of A1B1, which had the lowest yield of 9.78 t/hm2, the growing rate was 12.21%. In addition, A4B1 (nitrogen fertilizer 180 kg/hm2, planting density 20 hill/m2) and A3B2 (nitrogen fertilizer 150 kg/hm2, planting density 14.31 hill/m2) were higher yield combinations. The results reveal that the effect of nitrogen level and transplanting density on yield is significant, but the interaction is not significant. More abundant productive tillers, larger panicles and higher seed setting rate are the main determinants of high yield. When‘Shuyou 217’is planted in Chengdu Plain, the suitable planting density is 14.31 hill/m2 and the suitable nitrogen level is 150-180 kg/hm2.

  Effect of Sowing Dates on Soil Moisture and Yield of Winter Wheat 2018, 34 (29):  13-19.  DOI: 10.11924/j.issn.1000-6850.casb17070070 Abstract + ( 534 )   Under semiarid rain-fed condition, winter wheat‘Longzhong No.1’was used to study soil moisture and winter wheat yield under different sowing dates. The results showed that soil water content increased with the late-sowing of winter wheat. There were differences in soil moisture under different sowing dates and growth periods. Among the four sowing dates, the highest soil moisture was found in the latest sowing date (S4, October 11) and the lowest yield was found in the earliest sowing date (S1, September 21). The yield was similar between September 30 and October 11. On the whole, the soil moisture increased with the delay of sowing date. The late-sowing will not lead to the reduction of output.

  Effects of Sowing Dates and Planting Densities on Yield and Yield Components of Sugar Cane‘Yunzhe 05-51’with Early Maturing and High Sugar Content 2018, 34 (29):  20-25.  DOI: 10.11924/j.issn.1000-6850.casb17090089 Abstract + ( 880 )   In order to clarify the high yield and high efficiency cultivation technology of new sugarcane variety Yunzhe 05 - 51, the wide application of this variety in production was accelerated. The effect of sowing date and planting density on the yield and its components of sugarcane variety was studied in Longchuan get base in 2014 - 2016 at four different seeding time and four planting density treatments. The results showed that the sowing date had a significant effect on the stem diameter, effective stem number and yield of cloud 05 - 51", but had no significant effect on plant height and brix. The planting density had a significant effect on brix and cane yield of" Yunzhe 05 - 51". The average yield of sugarcane was as high as 150.70 t / hm 2 in January 20, and the difference of yield increase or decrease with the postponement of sowing date after January 20. When planting density B1 ( 150000 bud / hm2 ), the yield of sugarcane reached the highest value of 127.25 / hm 2, and the yield of sugarcane stem decreased with the decrease of planting density. Considering the interaction effect of sowing date and density, the best sowing date and planting density of " Yunzhe 05 - 51" were sown on January 20, and the planting density was B1 ( 150000 bud / hm 2 ).

  Populus euramericana cv.‘Hongxia’: Introduction and Cultivation Techniques 2018, 34 (29):  26-30.  DOI: 10.11924/j.issn.1000-6850.casb17100095 Abstract + ( 691 )   The paper aims to enrich the variety of colored-leaf trees in Beijing and expand the planting area of Populus euramericana cv‘. Hongxia’。The Populus euramericana cv‘. Hongxia’was introduced to Fangshan, Beijing from Chengdu, Sichuan, the phenological period observation, color change observation, cutting propagation, grafting propagation and the adaptability analysis of the plant to soil and light intensity were carried out. The results showed that: Populus euramericana cv.‘Hongxia’could complete the life cycle in Fangshan, Beijing; the survival rate of cutting propagation was over 80%, and the survival rate of grafting propagation was over 85%; it could grow in the refuse soil, the pasture soil and the architecture soil, but it grew in an undesirable way; it grew well in the nursery soil; the leaves were sensible to the stress reaction of the light intensity, and the colors of the leaves were bright; and there was the phenomenon of leaf scald, the leaf color may become green again and the leaves grew weakly; but the leaves grew well in the 75% of shading, and there was no phenomenon of leaf scald. The leaves of the tree could have the colorful effect of three colors: red orange, golden yellow and yellow green. In landscaping, Populus euramericana cv‘. Hongxia’can be planted in the plot of proper shading to achieve unusual colorful landscape.

  Diploid and Artificial Induced Homologous Tetraploid of Pteroceltis tatarinowii Maxim.: AFLP Analysis of Genetic Diversity 2018, 34 (29):  31-36.  DOI: 10.11924/j.issn.1000-6850.casb18020006 Abstract + ( 721 )   The paper aims to study the genetic diversity between diploid and homologous tetraploid of Pteroceltis tatarinowii Maxim chemically induced by colchicines from the DNA molecule level. We took the diploid and homologous tetraploid of Pteroceltis tatarinowii Maxim. as experimental materials and analyzed the DNA polymorphism and genetic distance by the AFLP molecular markers. The results showed that: there were significant differences in the DNA molecular genetic structure between homologous tetraploid induced by colchicines and diploid of Pteroceltis tatarinowii Maxim., and the DNA polymorphism of the diploid was higher than that of homologous tetraploid and the genetic distance between individual plants of tetraploid was higher than that of diploid in the whole level. The result provides a basis for further development of polyploid breeding and taking advantages of polyploid of Pteroceltis tatarinowii Maxim.

  Umbrella Affects Growth and Fruit Quality of Myrica rubra 2018, 34 (29):  37-41.  DOI: 10.11924/j.issn.1000-6850.casb17090158 Abstract + ( 559 )   Rain can cause much loss in fruit ripe season of bayberry. To improve fruit quality and economic benefit, umbrellas are applied to bayberry cultivation. The results showed that the average temperature under umbrella was 28.39℃, lower than that outside umbrella (CK) by 1.86℃. The average relative humidity under umbrella was 79.91%, higher than CK by 2.42 percentage points. The average length, width, thickness and content of chlorophyll of bayberry leaves under umbrella were lower than those of CK by 10.10%, 17.49%, 15.79% and 29.79%, respectively, and the average length of summer shoots were significantly lower than that of CK by 8.31%. The umbrella cultivation improved fruit quality, the averageb * value, hardness and soluble solids of treated fruits were higher than those of CK by 19.22%, 15.18% and 5.75% respectively, and the average acid content and fruit drop rate were lower than those of CK by 8.86% and 30.19% respectively. The picking time was longer than that of CK by 1-2 days, the economic fruit rate was 5.14% higher than that of CK, and the economic benefit was improved obviously. Thus, the umbrella cultivation technique can be applied to bayberry production.

  Study on Genetic Diversity of China Orchid Resources Using RAPD and ISSR Markers 2018, 34 (29):  42-47.  DOI: 10.11924/j.issn.1000-6850.casb17100075 Abstract + ( 555 )   The research aims at analyzing genetic relationship among China orchid in different areas, and providing reference for the resources exploitation and new variety breeding of China orchid at the molecular level .With RAPD and ISSR markers, the genetic diversity and genetic relationship of 21 cultivars of 7 species in China orchid were analyzed. The results showed that 96 clear bands were amplified with 39 RAPD and ISSR primers, 31 of these were polymorphic strips. UPGMA cluster analysis showed that the genetic distance ranged from 1.91 to 6.60 among 21 China orchid cultivars, the genetic distance of Cymbidium. goeringii was 1.91- 4.38, the genetic distance of C. ensifolium was 2.60- 6.20, the genetic distance of C. kanran Makino was between 2.20 and 5.80, the genetic distance of C. sinense was between 3.52 and 6.60, which showed higher genetic diversity. The results of cluster analysis were basically consistent with the traditional morphological classification results, indicating that genetic markers could reflect genetic resources diversity at the molecular level, with the advantages of high sensitivity and reliable results. It is concluded that there are some correlations between genetic relationship and geographical location among China orchid varieties and the study provide certain theory support for Cymbidium classification and genetic diversity research.

  Screening of Superior Individual Tea Plant Resources in Guzhang 2018, 34 (29):  48-54.  DOI: 10.11924/j.issn.1000-6850.casb18070045 Abstract + ( 534 )   This experiment aims to understand the changes and differences of biochemical components among the individual tea cultivars of Guzhang, judge the characteristics and suitability of each tea tree resource, and select a batch of excellent single plants matching the requirement of different teas. Using UV spectrophotometer method and high performance liquid chromatography (HPLC), we analyzed the main biochemical components (tea polyphenols, amino acids, soluble sugars, water extracts, caffeine, theanine, catechin) in fresh tea leaves in Guzhang. Based on the results, principal components analysis and cluster analysis were conducted. A total of 5 principal components were identified, which represented 86.90% of the biochemical components. Cluster analysis divided the 12 tea resources into 4 groups, in which the content of tea polyphenols and water extracts varied while the content of other component was close to each other. The whole performance of these tea resources had high water extracts, amino acids, soluble sugars, and low tea polyphenols, ratio of polyphenols to amino acid (TP/AA), caffeine. In the primary selection of 10 trial tea samples, 8 were supposed to be green tea resources, and the rest 2 were suitable for making both green and black tea. Besides, 3 specific resources with amino acids higher than 4% , 3 specific resources with tea polyphenols lower than 16%, 1 specific resource with caffeine lower than 2%, and 3 specific resources with TP/ AA lower than 5 were selected.

  Study and Application of Ornamental Green Manure in Sightseeing Agriculture 2018, 34 (29):  55-58.  DOI: 10.11924/j.issn.1000-6850.casb17100008 Abstract + ( 496 )   In the process of sightseeing fruit tea and garden construction, planting green manure crops with ornamental effects can beautify the rural environment, and it can also maintain soil and water, improve soil, and create a good ecological agricultural environment. At present, the research in this field was very weak, in this paper the concept of "ornamental green manure" was put forward and made a preliminary definition, the main role of ornamental green manure in agriculture tourism, configuration models were also discussed, in order to promote the application and development of ornamental green manure in the construction process of ornamental fruit and tea garden and ecological village.

  Evaluation of Soil Physical Properties of New Green Spaces Based on Fuzzy Mathematical Method 2018, 34 (29):  59-63.  DOI: 10.11924/j.issn.1000-6850.casb17120118 Abstract + ( 565 )   Physical properties of soil in urban green spaces (UGS) have great influence on ecosystem functions. To study physical properties of different soil levels of green spaces, soil samples in Xi’an Botanic Garden were collected, six indexes were chosen, including bulk density, total porosity, capillary porosity, non- capillary porosity, water holding capacity and soil moisture content, Q value of soil samples were evaluated with fuzzy mathematical method, SPSS17.0 and Excel. The results showed that Q values of soil samples of topsoil (0-25 cm), surface soil (25-50 cm), middle soil (50-70 cm) and deep soil (70-90 cm) were 21.69, 61.24, 71.19, 66.89, which indicated that they were in extremely low level, lower level, good level and lower level, respectively. The average Q value was 55.25, which indicated that the soil was in lower level. The reason of low Q value in topsoil and surface soil was that the soil was repeatedly rolled by heavy machinery during the construction. The effect on middle soil was lower. The Q value of deep soil was lower than that of middle soil along with the increase of soil depth. The comparison of the weight of the indexes showed that bulk density was the main index affecting the physical property. The bulk density decreased with the increase of soil depth. However, the weight of natural water content increased with the increase of soil depth.

  Agricultural Soil Nutrients Evaluation and Analysis: Dali County 2018, 34 (29):  64-68.  DOI: 10.11924/j.issn.1000-6850.casb17120127 Abstract + ( 715 )   The authors analyzed pH value, organic matter, total nitrogen, alkaline hydrolysis nitrogen, available phosphorus and available potassium of 490 agricultural soil samples collected in Dali County. The data were statistically summed up by Microsoft Excel 2010. The results showed that agricultural soil in Dali County were mainly alkaline. The content of organic matter was moderately deficient and the total nitrogen was slightly deficient. The content of alkaline hydrolysis nitrogen was deficient; available phosphorus was moderately low; available potassium was moderately rich. It is concluded that the soil fertility could be improved by changing soil pH value, increasing the inputs of nitrogen and phosphorus, thus to improve the crop yield and quality.

  Relationship Between Soil Fungal Community Structure and Physical and Chemical Properties of Different Seasons Swamp Meadow in Napahai Wetland 2018, 34 (29):  69-75.  DOI: 10.11924/j.issn.1000-6850.casb17080110 Abstract + ( 517 )   To clarify the seasonal variation rule of soil fungal community structure and its relationship with physical and chemical properties in the swamp meadow of Napahai wetland, we took the method of diluted culture combined with morphological identification comparative analysis, to study the seasonal variation of soil fungal community structure in dry season and rainy season, and explored its relationship with physical andchemical properties. The results showed that (1) soil organic matter, total nitrogen, available nitrogen, available potassium and available phosphorus in 0-20 cm soil layer in rainy season were higher than those in dry season, but those in 20-40 cm soil layer in dry season were higher than those in rainy season, except total nitrogen; the soil bulk density and natural water content in 0-20 cm and 20-40 cm soil layer in rainy season were higher than that in dry season; (2) soil fungal diversity had significant seasonal variation, except Pielou index (JSW ), fungi number, diversity index (H '') and Margalef index (DMA ) in dry season were higher than those in rainy season of 0-20 cm soil layer, diversity index (H'' ) and Margalef index(DMA ) in dry season were higher than those in rainy season in 20-40 cm soil layer and fungi number and Pielou index (JSW ) in rainy season were higher than those in dry season; (3) morphological identification showed that: 12 genera and 11 genera of fungi were isolated in the dry season and rainy season respectively, and the community structure was similar in composition, but also had some differences,Aspergillus andPythium belonged to the dominant species in the dry season and the rainy season, Cladosporium and Cytospora Ehrenb were the genera of dry season, and Neurosporacrasa was endemic to rainy season; (4) Pearson correlation analysis showed that: soil organic matter, total nitrogen, available nitrogen, available potassium, available phosphorus, bulk density and natural water content were closely related to soil fungal community structure in dry season and rainy season. There were obvious seasonal variation characteristics of soil fungal diversity and community composition in different seasons, soil physical and chemical properties and fungal community structure were closely related, and the results provided references for studying the influence of fungal community on Napahai wetland ecosystem.

  Effect of a Disinfectant on Greenhouse Watermelon Growth and Soil in Eastern Guanzhong of Shaanxi 2018, 34 (29):  76-81.  DOI: 10.11924/j.issn.1000-6850.casb17100052 Abstract + ( 687 )   Weinan is a major watermelon cultivation area in Shaanxi. However, continuous cropping and mismanaged field practices have led to local soil deterioration, which causes intensified diseases and pests attack and considerable watermelon quality decrease. To overcome this problem, a disinfectant is applied to local main cultivar, mini watermelon genotype‘Linglongwang’, to explore the effect on greenhouse watermelon growth, incidence of root-knot nematode, microorganism and soil enzymes. The results showed that the plant height and stem diameter increased with the increase of disinfectant concentration in the later growth stage of plant. Total fresh weight, yield and soluble solids all reached the highest under the disinfectant concentration of 52.5 kg/hm2. The yield per hectare also increased by 7.30% compared with that of CK. Moreover, the incidence of root-knot nematode decreased significantly with the increase of disinfectant level, the correlation coefficient was 0.7475. Soil total bacterial count decreased in the 0-10 and 10-20 cm soil layers after the addition of disinfectant, while the activities of four soil enzymes increased compared with that of CK. The activities of sucrase and alkaline phosphatase were also the highest at 52.5 kg/hm2 level of disinfectant application.

  Evaluation of Soil Quality and Heavy Metal Safety in Tea Garden of 13 Towns in Yingde, Guangdong 2018, 34 (29):  82-91.  DOI: 10.11924/j.issn.1000-6850.casb17090012 Abstract + ( 704 )   To investigate soil quality and heavy metal safety in tea growing areas in Yingde, Guangdong, the authors collected 76 tea garden soil samples from 13 towns and measured soil pH value, fertility index and 6 kinds of heavy metals included chromium, copper, lead, arsenic, cadmium and mercury according to national standards, and further evaluated the heavy metal safety. The results showed that there were 10 areas which had the phenomenon of acidification or basification, accounting for 13.15% of the total soil samples; among those samples, Shakou and Yunling samples were acidated, Dawan, Shigutang and Shihuipu samples were alkalized. The analysis of soil fertility showed that most samples located in the lower position of ± 2, relatively concentrated, indicating that the majority of Yingde tea garden soil had lower fertility. According to single heavy metal pollution index (Pi ), arsenic pollution index of 9.21% soil samples exceeded the standard, Hg and Cr pollution index of 1.31% soil samples exceeded the standard. The comprehensive pollution indexes (P ) of most soil samples were less than 0.70, which were at safe level. Heavy metal pollution of 3.95% soil samples (Wangfu, Shihuipu and Yinghong) reached the alert level; and 1.31% (Donghua) was in light pollution grade. The study shows that soil in Yingde is in lower fertility, with the phenomenon of acidification and basification, and a small amount of tea garden soil has heavy metal pollution.

  Climate Suitability of Chestnut in Tianshui 2018, 34 (29):  92-97.  DOI: 10.11924/j.issn.1000-6850.casb18070066 Abstract + ( 531 )   Based on chestnut’s phenology observation data and meteorological data from 1971 to 2017 at Maiji National Base Weather Station, the model for assessing the climate suitability degree of chestnut was set up. We analyzed the climate suitability of every main growth periods of chestnut. The results showed that chestnut synthesize climate suitability degree was 0.67, sunshine suitability degree was 0.74, water suitability degree was 0.66 and temperature suitability degree was 0.65 from 1971 to 2017. The synthesize climate suitability degree increased with time in recent years. The water and temperature suitability degree varied with different growth periods and sunshine suitability degree kept high value in all growth periods. The variation range of temperature suitability degree was big and water suitability degree had a rising trend in sprout and flowering period. The water suitability degree was low while temperature suitability degree was high in fruit expansion period, while the temperature suitability degree was low and the water suitability degree was high in fruit maturation period, the environment condition of growth for chestnut become more unsuitable. We suggested that water supplying in sprout and flowering period and heat resource in maturation period should be considered in building chestnut orchard.

  Growth Factors and Signaling Pathway Affect Hair Follicle Development: A Review 2018, 34 (29):  98-103.  DOI: 10.11924/j.issn.1000-6850.casb17080068 Abstract + ( 694 )   The development of hair follicle (HF) is cyclical, and its cyclogeny is regulated by many factors. The growth factors such as VEGF, HSP and EGF can affect the growth and development of hair follicles in a direct or indirect way. Some signaling pathways, such as Wnt and BMP/TGF beta also regulate hair follicle cycle and other life activities. We summarized the research progress on the regulators for HF development, including growth factors, hormones, active proteins and signal pathways. Finally, we put forward the potential research hotspot and application prospect of HF development.

  Effects of Tea Saponin on Rumen Methanogens and Methane Emission in Dairy Cows 2018, 34 (29):  104-111.  DOI: 10.11924/j.issn.1000-6850.casb17080108 Abstract + ( 522 )   We aim to investigate the effect of tea saponin (TS) on the rumen methanogens and methane emission in dairy cows by in vitro fermentation and in vivo experiments. For the in vitro fermentation model, the 0.5 g total mixed ration (TMR) was used as the substrate, the concentration gradient of added TS was 0 (control), 5, 10 and 15 g/L. For the in vivo experiment, 4 healthy Holstein dairy cows with permanent rumen fistula were fed with TMR containing 0 (control), 15, 30 and 45 g/d TS, respectively. The experiment lasted for 4 periods (each period lasted for 20 days, including 7 days of pre-feeding and 14 days of test). The total number and population structure of methanogens in rumen were determined by collecting the rumen fluid and extracting the genomic DNA, and then calculating the gene copy number of methanogens and other strains. The results showed that the addition of TS had no significant effect on the total number of methanogens in the in vitro fermentation model (P>0.05), but it could significantly change the population structure of methanogens. The numbers of M. formicium and M. smithii significantly decreased (P<0.05), the number of M. stadtmanae significantly increased (P<0.05), and the number of M. ruminantium did not change significantly (P>0.05). In the in vitro fermentation model, compared with the control group, the high addition of TS (10 and 15 g/L) could significantly reduce the gas production (P<0.05) and the fermentation rate (P<0.01), decrease the number of protozoa (P<0.01), and inhibit methane emission (P<0.01). However, in the in vivo experiment, the above results were not significantly different (P>0.05). In conclusion, the in vitro experiment shows that the addition of TS in the diet cannot change the total number of methanogens, but can change the population structure of methanogens to a certain extent, reduce the number of protozoa, decrease the gas production, achieving the methane emission reduction. Whereas the in vivo experiment indicates that the addition of TS has no significant effect on rumen methanogens in dairy cows.

  The Validation Research on Interaction Between PRRSV 2b Protein and LGALS1 2018, 34 (29):  112-117.  DOI: 10.11924/j.issn.1000-6850.casb17090090 Abstract + ( 472 )   The aim is to study the interaction between porcine reproductive and respiratory syndrome virus (PRRSV) 2b protein and host cell proteins. In this study, we used yeast regression test, GST-pull down assay and co- immunoprecipitation assay to verify the interaction between LGALS1 and PRRSV 2b. The results showed that in the yeast regression test, hybrid yeast strain of LGALS1 and2b gene grew blue yeast colonies on SD/-Trp-Leu-His-Ade/AbA/X-α-gal plates. In the GST-pull down assay, the fusion protein bands were detected by Western blot in the samples reacted with 2b protein and LGALS1. In the co-immunoprecipitation assay, samples reacted with 2b protein and LGALS1 were precipitated with HA monoclonal antibody and the corresponding protein bands were detected by Western blot. This paper demonstrates the interaction between LGALS1 and PRRSV 2b protein and provides a theoretical basis for studying PRRSV infection and proliferation.

  Effects of Pollination Methods on Pollination of Seed Lotus 赵东绪,, and 2018, 34 (29):  118-122.  DOI: 10.11924/j.issn.1000-6850.casb17080038 Abstract + ( 699 )   Seed Lotus, a plant with low basal yield, is dependent on the activities of pollinators to complete ovule fertilization, and the quality of pollination is an important factor that directly affects the yield and quality of the lotus.[Method]Apis cerana Fabricius and Italy bees are the ideal pollinators. Each has its unique biological characteristics, and has differences in biological characteristics, such as stress resistance, collection activity and collection preference. According to Pollinate the lotus seeds with Apis cerana Fabricius and Italy bees, we makes a comparative study between the acquisition activities and the effect of pollination. [Result]The results of activity mode showed that their collection behavior was similar, but the activity patterns were different. During the summer season, the Italy bees work longer than Apis cerana Fabricius, so the Italy bees had higher pollination efficiency. And the results of pollination effect show that there was no significant difference between the Apis cerana Fabricius group and Italy bees group in the 4 indicators, Seed Lotus yield which related single grain weight, seed grafting rate, vertical and horizontal diameter of lotus seeds related to commodity, but significant difference was noted between natural pollination group and entomophily-free group.The average seed picking of Italy bees is the highest rate of 87.82%, which seed rate were increased by 33.27% and 79.10% compared with natural pollination group and entomophily-free group. The lotus seed average single grain weight of the former group is up to 3.68g, significantly higher than any other types of pollination, and lotus fruit type of entomophily-free group is minimum.What’s more, there is a marked difference noted in yield and related sub grain weight between Italian bee pollination group and other groups. Compared with the Chinese, natural’s and entomophily-free group, the production of Italian has increased respectively by 25.88%、37.67% and 665.50%.[Conclusion]For the reasons given above, the pollination effect of Apis cerana Fabricius is similar to that of Italy bees. Both can complete the tasks of pollination. Italian can obtain higher Seed Lotus yield, however, and it is superior to natural pollination and entomophily-free pollination, resulting in higher efficiency of pollination.

  Crop Germplasm Resources in Fujian: Questionnaire Investigation and Information Sharing Utilization 2018, 34 (29):  123-130.  DOI: 10.11924/j.issn.1000-6850.casb17120061 Abstract + ( 453 )   Investigation on Crop Germplasm Resources and Their Sharing Utilization in Fujian Huang Aiping, Zhang Mei, Lin Haiqing, Ke Wenhui (Institute of Agricultural Economics and Scientific Information, Fujian Academy of Agricultural Sciences, Fuzhou 350003) Abstract: [Objective] This paper aims to study the research and utilization status of crop germplasm in Fujian. [Method]The crop germplasm resources and their sharing utilization in 9 provincial and 7 prefectural (city-level) scientific research units (extension centers) of Fujian Province were analyzed by questionnaire investigation. [Result]The results showed that the quality of research personnel was generally high, 64.78% of which worked on germplasm resources. The most attention of researchers were paid on quality of germplasm and yield traits. The main ways for researchers to get germplasm resources were inter-unit germplasm exchange and self-collection through literature information; 75.03% of researchers thought that the main factors restricted the development of crop germplasm study were insufficient fund, poor experimental conditions and lack of collaboration mechanisms. Two countermeasures for optimizing the sharing utilization of crop germplasm resources were considered to be: enhancing the scientific research cooperation and exchange, and improving the sharing level. [Conclusion]The current crop germplasm resources sharing service didn’t match with the research needs. Therefore, based on the local germplasm resources in Fujian, it was suggested to collect and integrate Fujian crop germplasm resources data, and construct Fujian germplasm resources information sharing and managing platform in order to realize the sharing of crop germplasm resources, and improve the resource utilization efficiency.

  Comparative Analysis of Muscle Fatty Acid in Different Growth Stages of Wild and Cultured Mastacembelus armatus 2018, 34 (29):  131-136.  DOI: 10.11924/j.issn.1000-6850.casb17070118 Abstract + ( 558 )   In order to compare the fatty acid composition in muscle of Mastacembelus armatus between farmed and wild populations, by gas chromatography analysis of cultured and wild fish, Mastacembelus armatus at juvenile ,fingerling and adult stage ,the muscle fatty acid composition and relative content were detected. The results showed that the muscle of cultured and wild Mastacembelus armatus had 24 kinds of fatty acids, the content of unsaturated fatty acid were the highest, the average content respectively (65.63 + 1.88)% and (59.03 + 2.11)%, especially rich in C18:2, docosahexaenoic acid(DHA) eicosapntemacnioc acid(EPA) and arachidonic acid(AA) polyunsaturated fatty acids; ΣUFA/ΣSFA ratio from juvenile to adult stage was on the rise, and the cultured population (2.45 + 0.21) higher than that of wild populations (1.98 + 0.15); the ratio of P/S to 1 for breeding Mastacembelus armatus at adult stage, more than half of the freshwater fish. Comprehensive analysis showed that the fatty acid content in muscle at different growth stages of farmed and wild Mastacembelus armatus although had some differences,also the breeding Mastacembelus armatus Sadvantage over wild Mastacembelus armatus but the meat quality in breeding Mastacembelus armatus fatty acid composition and equilibrium Similar to wild Mastacembelus armatus .

  Development Evaluation of Agricultural Circular Economy in Sichuan 何鹏 2018, 34 (29):  137-142.  DOI: 10.11924/j.issn.1000-6850.casb17120075 Abstract + ( 603 )   Sichuan is a major agricultural province, the problems of non-point source pollution and resource shortage are particularly prominent. It is urgent to carry out the evaluation of agricultural circular economy. Based on the BPEIR conceptual model, and combined with the Delphi method (Delphi) and the main analytic hierarchy process (AHP), we screened 17 factors for comprehensive development index and obstacle analysis. The results show that Sichuan’s agricultural circular economy development index has an overall rising trend; the main obstacle factor affecting the agricultural circular economy in Sichuan is resource input reduction, single index obstacle degree of 6 factors is in an order of fertilizer use intensity, agricultural film use level, pesticide use level, the per capita arable land area, grain yield per capita, and cropping index. Combined with the research results, we put forward 4 suggestions: to grasp the good momentum, making out the local ecological agriculture development plan in accordance with the national policies; to focus on obstacle factors restricting the agricultural circular economy, strengthening weak links of resource input reduction; to enhance agricultural ecological resources protection, securing agricultural land area and promoting the productivity of farmland; to improve the training of farmers and cultivate new business entities, developing the profit mechanism of farmers’income.

  Implementation Effect of Agricultural Subsidy Policy: Review and Prospect 2018, 34 (29):  143-150.  DOI: 10.11924/j.issn.1000-6850.casb18050070 Abstract + ( 538 )   To understand the research status quo of implementation effect of agricultural subsidy policy in China, grasp the research results and existing deficiencies, and then point out the direction of future research on the effect of agricultural subsidy policy, and provide a basis for the adjustment and perfection of agricultural subsidy policy, using the literature research method, the research on the effect of agricultural subsidy policy at home and abroad is reviewed from two perspectives: research perspective and research method. It is found that the research on the effect of agricultural subsidy policy in foreign literature can be mainly divided into economic effects research, social effects research and ecological effects research. The domestic literature mainly focuses on the economic effects research, studies on social effects and ecological effects are secondary, the research on ecological effects is mostly for qualitative research and policy recommendations. Based on this, three aspects of the research can be drawn: firstly, the research perspective is diverse but not comprehensive enough; secondly, the research methods are diversified and more perfect; thirdly, the analysis of policy economic effects is comprehensive but the field of vision is narrow; the research on social effects and ecological effects are not enough. In the end, according to the characteristics of the existing literature, the prospect of research on the effect of agricultural subsidy policy is discussed, which can be summarized as ecologicalization, modernization and internationalization.

  Spatial Distribution Rules of Poor Villages in Wulingyuan District, Zhangjiajie City 2018, 34 (29):  151-156.  DOI: 10.11924/j.issn.1000-6850.casb17110088 Abstract + ( 487 )   The exploration of spatial distribution rules of poor villages in Wulingyuan District, Zhangjiajie City aims at accelerating the poverty alleviation process and the pro-poor tourism development. Three methods of overlay analysis, buffer analysis and density analysis in GIS spatial analysis were applied to study the spatial distribution rules of 31 poor villages in this region based on data of early 2016. The result indicates that: the scenic spots and township governments have strong radiation effect, the number of poor villages increased along with the distance away from the scenic spots and township governments; poor villages are mainly distributed along the ways to the scenic spots; however, the arterial traffic have weak effect on poverty alleviation; poor villages are concentrated in places with weak economy while scattered in those areas with better economy. Therefore, the targeted poverty alleviation in tourism regions should focus on the leading and radiation role of the scenic spots, improving the infrastructure construction and enhancing the function of government agencies.

  Rural Interest-linked Mechanism Based on "CPC Branch +" Platform: A Case Study of Tangling Village, Jurong City, Jiangsu Province 2018, 34 (29):  157-164.  DOI: 10.11924/j.issn.1000-6850.casb18010171 Abstract + ( 613 )   Grass- roots Party organizations are the most important leading force in rural areas. The development of rural economy should take advantage of the leadership, cohesion and coordination of the Party organizations. Although great achievements have been made in China''s rural economy, the peasants are still poor, the collective economic strength is still weak and the economic leadership of the grass- roots Party organizations is still insufficient. Based on the history of grass-roots Party branches in China, and the practice of economic development of the Party organizations in some places, this paper puts forward the tentative plan of the mechanism of interest connection with the core of“CPC Branch +”, and carries on the concrete analysis for the“CPC Branch+”of Tangling village. The study finds that Tangling village grows fast through the "CPC Branch + " platform of interest- linked mechanism. The local villagers are highly satisfied with the Party organizations and the economic development effect. "CPC Branch +" platform of interest-linked mechanism provides a new path for rural economic development. The "CPC Branch + " platform of interest- linked mechanism can integrate all aspects of resources to develop the economy, and stimulate business entities to participate in industrial activities; the economy can therefore be developed. What’s more, through the Party organization to determinate the nature and development direction of the village industry, the results of economic development will benefit the masses of farmers and achieve the purpose of rural revitalization.