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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (12): 159-163.doi: 10.11924/j.issn.1000-6850.2011-3247

所属专题： 生物技术； 玉米

• 生物技术科学 • 上一篇    下一篇

玉米NAC转录因子片段的克隆及分析

王彦威 潘金豹 韩俊 张秋芝 南张杰 孙清鹏   

  • 收稿日期:2011-11-04 修回日期:2011-12-29 出版日期:2012-04-25 发布日期:2012-04-25

Cloning and Sequence Analysis of NAC Transcription Factor Gene Fragment in Maize (Zea mays)

  • Received:2011-11-04 Revised:2011-12-29 Online:2012-04-25 Published:2012-04-25

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摘要：

以三叶期玉米幼苗为材料，根据NAC转录因子特有的保守结构设计特异引物，利用RT-PCR技术获得一个玉米NAC转录因子的基因片段，并对该片段进行了测序和生物信息学分析。测序分析结果表明，克隆得到的NAC转录因子基因片段为230 bp，聚类分析结果表明其属于NAC家族的NAM亚类。该片段的获得为克隆玉米NAC转录因子基因全长，进而为玉米抗逆育种奠定了基础。

关键词: 植酸酶, 植酸酶, 生产, 应用

Abstract:

In this study, maize (Zea mays) seedlings with three leaves were used as experimental materials, a NAC transcription factor gene fragment was cloned and sequenced. The specific primer was designed according to the specific conserved domain of NAC transcription factor, and a gene fragment was amplified by RT-PCR. The results showed that, the fragment was 230 bp, and belonged to the NAC family NAM subfamily. The fragment laid the foundation for cloning the full-length of maize NAC transcription and stress-resistant breeding in future.