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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (16): 186-189.doi: 10.11924/j.issn.1000-6850.2011-3733

所属专题： 资源与环境

• 林学 园艺 园林 • 上一篇    下一篇

枇杷种质资源SSR标记指纹图谱的构建

岳娜 胡倩倩 方星 邱学林 储春荣 祁红丽 孟祥勋   

  • 收稿日期:2011-12-09 修回日期:2012-02-25 出版日期:2012-06-05 发布日期:2012-06-05
  • 基金资助:

    江苏省高新技术项目

Fingerprinting Patterns of SSR Markers Constructed for 33 Loquat [Eriobotrya japonica (Thunb.) Lindl.] Germplasms

  • Received:2011-12-09 Revised:2012-02-25 Online:2012-06-05 Published:2012-06-05

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摘要：

为构建枇杷种质资源指纹图谱，采用SSR标记技术对国家枇杷种质资源圃33份品种进行多态性分析，从近缘属苹果属和梨属的51对SSR引物中筛选出能够稳定扩增，多态性好的7对引物，Hi01d05F、Hi12f04F、CH02B10F、Hi03a03、Hi03e04、Hi04a05和Hi02c07来构建指纹图谱。结果表明，应用这7对引物可100%区别33个枇杷种质。这种SSR标记构建的枇杷种质资源指纹图谱对于品种分类及鉴定具有重要的应用价值。

关键词: 研究, 研究

Abstract:

SSR sequences are the reproducible, stable, rich polymorphism DNA molecular markers. Fingerprint patterns of the SSR markers for 33 loquat varieties in national loquat germplasm resource garden were constructed by specifically amplifying polymorphism from 7 pairs of primers screened from 51 pairs of primers of the relatives Malus and Pyrus. The results showed that, the uses of seven primers, Hi01d05F, Hi12f04F, CH02B10F, Hi03a03, Hi03e04, Hi04a05 and Hi02c07, could distinguish 33 loquat germplasm from one another, completely, providing an essential criterion for classification and identification of the loquat resources.