欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (13): 163-166.doi: 10.11924/j.issn.1000-6850.2012-0032

所属专题： 园艺

• 林学 园艺 园林 • 上一篇    下一篇

利用分子标记鉴定辣椒抗疫病材料

郭爽 黄贞 常绍东 刘玉平 曹翠文   

  • 收稿日期:2012-01-04 修回日期:2012-03-01 出版日期:2012-05-05 发布日期:2012-05-05
  • 基金资助:

    广州市农科院院立项目

Identification of Resistance to Phytophthora Blight in Hot Pepper Using Molecular Marker

  • Received:2012-01-04 Revised:2012-03-01 Online:2012-05-05 Published:2012-05-05

PDF (PC)

771

摘要：

为了利用分子标记快速准确鉴定辣椒抗疫病材料，以36份辣椒高代自交系为材料，利用与辣椒疫病紧密连锁的2对标记引物，结合田间抗疫病性调查，鉴定辣椒抗疫病种育材料。实验结果表明，26份材料的2个标记引物的分子标记鉴定结果与田间调查结果完全吻合，包括22份抗病材料，4份感病材料；3份材料的分子鉴定结果与田间调查结果相反；4份材料在2对引物上的鉴定结果不同，这些差异可能是由于标记所连锁的抗性位点不同、抗性基因的表达形式不同造成的。

关键词: 生化成分, 生化成分

Abstract:

In order to identify resistance to phytophthora blight of hot pepper quickly and accurately, the phytophthora blight resistance of 36 hot pepper materials was identified by two molecular markers closely linked with resistance genes and field surveys. Identification results by molecular markers completely consistent with the results of field surveys on 26 materials, including 22 resistant materials and 4 susceptible materials. 3 materials had the opposite identification results by two ways. 4 materials had the different results in two markers. These differences may be caused by the diversity of resistance loci and diverse expression of resistance genes.