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中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (18): 245-250.doi: 10.11924/j.issn.1000-6850.2013-2589

• 农学 农业基础科学 • 上一篇    下一篇

不同分散温度、时间对双波长法测定高粱直、支链淀粉及总淀粉含量的影响

刘选选 曹国璠 任明见 张君 李祥栋 陈杏   

  • 收稿日期:2013-10-07 修回日期:2013-10-24 出版日期:2014-06-25 发布日期:2014-06-25
  • 基金资助:
    农业部引进国际先进农业科学技术项目“基于茅台酒用高粱的种质资源引进及其良种选育”(2012-Z37)；科技部农业科技成果转化资金项目“良种繁育及示范推广”(2013GB2F200437)；贵州省科技厅的工程中心建设计划项目“贵州省高粱工程技术研究中心建设项目”(黔科合农G字[2012]4004号)。

The Effect of Different Sample Dispersion Temperatures and Time on Sorghum Amylose and Amylopectin, Total Starch Content Determination by Double Wavelength Spectrophotometric Method

  • Received:2013-10-07 Revised:2013-10-24 Online:2014-06-25 Published:2014-06-25

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摘要： 为了测定高粱直、支链淀粉及总淀粉含量，采用双波长比色法研究了不同分散温度、时间处理对高粱淀粉直、支链淀粉及总淀粉含量的影响。结果表明，在试验温度70~100℃范围内，75℃分散15 min时支链淀粉百分含量最高，85℃分散5 min时支链淀粉百分含量最低。对于直链淀粉，95℃以后，随着分散的时间延长，测得的高粱直链淀粉百分比不断增高。样品分散温度与时间对高粱中总淀粉含量影响与支链淀粉相同，其测定值在75℃分散15 min时最高。由此可知，不同分散温度、时间处理对双波长法测定高粱淀粉直、支链淀粉及总淀粉含量影响不一样。

关键词: 土壤肥力, 土壤肥力

Abstract: In order to determine the amylose and amylopectin, total starch content of sorghum, the effect of different sample dispersion temperatures and times on Sorghum amylose and amylopectin, total starch content by double wavelength spectrophotometric method were studied by double wavelength spectrophotometric method. In the range of 70-100℃, the percentage of amylopectin was the highest when the sample dispersion temperature and time were 75℃ and 15 min, the percentage of amylopectin was the lowest, among the sample dispersion temperature and time were 85℃ and 5min. For the amylose, the longer of dispersion, the higher of percentage, after 90℃. When the sample dispersion temperature was 75℃, the determined total starch content was the highest. These results demonstrated that the effects of sample dispersion temperatures and times on the determination of amylose, amylopectin and total starch content were different.