辣椒轻斑驳病毒(PMMoV)新疆加工型辣椒分离物的鉴定和致病型分析

doi:10.11924/j.issn.1000-6850.2014-0836

中国农学通报 ›› 2014, Vol. 30 ›› Issue (25): 296-302.doi: 10.11924/j.issn.1000-6850.2014-0836

所属专题：园艺

辣椒轻斑驳病毒(PMMoV)新疆加工型辣椒分离物的鉴定和致病型分析

张强张春竹罗明李克梅

收稿日期:2014-03-25 修回日期:2014-05-27 出版日期:2014-09-05 发布日期:2014-09-05
基金资助:
新疆维吾尔自治区科技成果转化专项资金项目“辣椒新品种繁育、高效栽培技术示范及系列产品开发和加工”(201154106)；新疆维吾尔自治区研究生科研创新项目“新疆加工型辣椒种子带毒的分子检测及种子脱毒技术研究”(XJGRI2013107)。

Identification and Pathotype Analysis of Pepper Mild Mottle Virus in Processing Pepper from Xinjiang

Received:2014-03-25 Revised:2014-05-27 Online:2014-09-05 Published:2014-09-05

摘要/Abstract

摘要： 为明确侵染新疆南疆巴州加工型辣椒主产区的辣椒轻斑驳病毒(PMMoV)的致病型，利用双抗体夹心酶联免疫吸附法(DAS-ELISA)、反转录聚合酶链式反应(RT-PCR)和基因克隆、序列分析对南疆巴州加工型辣椒主产区的辣椒病样进行PMMoV的检测及其致病型鉴定。结果表明，从辣椒植株、椒果及种子样品中均检测到PMMoV。通过对预期576 bp大小的3个PMMoV扩增产物进行克隆、测序和序列分析表明，PMMoV新疆加工型辣椒分离物CP基因的核苷酸和氨基酸序列与已报道的PMMoV分离物具有较高同源性，分别为89.0%~99.2%和95.5%~100.0%；其中，与PMMoV以色列分离物EF434393同源性最高，PMMoV新疆各分离物之间CP基因高度同源。依据CP基因序列及系统发育分析将PMMoV新疆加工型辣椒分离物划分为P_1,2致病型。

关键词: 研究, 研究

Abstract: To identify the pathotype of PMMoV infecting processing pepper in Xinjiang, some disease samples collected from southern Bavaria were detected by double-antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISA) and identified with reverse transcription-polymerase chain reaction (RT-PCR), cloning and sequence analysis. The results showed that PMMoV was detected from pepper plants, pepper fruit and seed samples. Three target amplified fragments with sizes of 576 bp were cloned, sequenced and analyzed. The results indicated that the similarity of the nucleotide and deduced amino acid sequences of CP gene showed highly identity between PMMoV–XJ isolates and that have been reported, sharing of 89.0%- 99.2% and 95.5%- 100.0% sequences identity respectively, and was most closely related to PMMoV- Israel strain EF434393. High sequence identities of CP gene were showed among Xinjiang isolates. Phylogenetic analysis based on the sequences of CP gene, the PMMoV isolates in Xinjiang processing pepper were belonged to P_1,2 pathotype.

张强张春竹罗明李克梅. 辣椒轻斑驳病毒(PMMoV)新疆加工型辣椒分离物的鉴定和致病型分析[J]. 中国农学通报, 2014, 30(25): 296-302.

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辣椒轻斑驳病毒(PMMoV)新疆加工型辣椒分离物的鉴定和致病型分析

Identification and Pathotype Analysis of Pepper Mild Mottle Virus in Processing Pepper from Xinjiang

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