棘孢木霉发酵液对番茄早疫病抗氧化系统和脂质过氧化作用的影响

doi:10.11924/j.issn.1000-6850.casb16030180

中国农学通报 ›› 2017, Vol. 33 ›› Issue (5): 90-94.doi: 10.11924/j.issn.1000-6850.casb16030180

所属专题：生物技术；园艺

棘孢木霉发酵液对番茄早疫病抗氧化系统和脂质过氧化作用的影响

杨帅,孙丽丽,邓世林,袁碧玉,苏佳满,王志英,孙卉

东北林业大学,东北林业大学,东北林业大学,东北林业大学,东北林业大学,东北林业大学,海林市林业局

收稿日期:2016-03-24 修回日期:2017-01-05 接受日期:2016-06-20 出版日期:2017-03-01 发布日期:2017-03-01
通讯作者: 王志英
基金资助:
东北林业大学本科生创新项目“绿色木霉可湿性粉剂研制及其杀菌活性测定”(201310225185)。“绿色木霉可湿性粉剂研制及其杀菌活性测定”(201310225185)。

Effects of Trichoderma asperellum Fermentation Broth on Antioxidant System and Lipid Peroxidation of Alternaria solani Sorauer

Received:2016-03-24 Revised:2017-01-05 Accepted:2016-06-20 Online:2017-03-01 Published:2017-03-01

摘要/Abstract

摘要： 为了初步探究棘孢木霉(Trichoderma asperellum)发酵液对番茄早疫病菌的抗氧化系统及细胞膜脂质过氧化作用的影响。本文以番茄早疫病菌(Alternaria solani)为靶标菌,以棘孢木霉(Trichoderma asperellum)发酵液为材料,测定了6~30 h内番茄早疫病菌防御酶(超氧化物歧化酶SOD、过氧化氢酶CAT、过氧化物酶POD)的活力、还原型谷胱甘肽(GSH)和丙二醛(MDA)含量及电导率的变化规律,初步分析了棘孢木霉发酵液对番茄早疫病菌抗氧化和菌体脂质过氧化作用的影响。结果表明,棘孢木霉发酵液处理和对照组的番茄早疫病菌MDA含量均呈上升趋势,且处理组高于对照组,为对照的3.10～4.03倍。与对照相比,棘孢木霉发酵液处理组番茄早疫病菌的CAT、SOD、POD活力及GSH含量均呈不同程度的下降,CAT、SOD、POD的活力降低率范围分别为38.99 ~ 55.51%、9.00 ~3 7.11%、34.70 ~ 55.84%；GSH的含量降低率范围为5.33 ~ 81.36%。棘孢木霉发酵液处理组导致番茄早疫病菌电导率随处理时间的延长而升高,30 h电导率高于对照的92.41%。棘孢木霉发酵液降低了番茄早疫病菌抗氧化防御酶的活力和GSH含量,增加了MDA的含量,同时其电导率显著升高,从而抑制番茄早疫病菌的生长发育。

关键词: 水产养殖, 水产养殖, 正规贷款, 差异性

Abstract: The activities of defensive enzymes in Alternaria solani including SOD, CAT, POD, and the GSH contents and changes of conductivity of MDA were analyzed during the period of 30 h under Trichoderma asperellum fermentation broth stress, in order to study the effects of T. asperellum broth on defensive enzymes and lipid peroxidation of A. solani. The results showed that the contents of MDA of the treated and control groups of A. solani both increased; and the contents of MDA among the treated groups were 3.10-4.03-fold as high as that of the control groups. Being treated with T. asperellum, the activities of CAT, SOD and POD of A. solani, and the contents of GSH in A. solani all decreased in different degrees compared with that of the control groups. The CAT, SOD and POD activities were decreased by 38.99%-55.51%, 9.00%-37.11% and 34.70%- 55.84% , respectively. The contents of GSH were decreased by 5.33%- 81.36% . Being treated with T. asperellum fermentation broth, the conductivity rate of A. solani increased with the increase of treatment time; the conductivity rate was increased by 92.41% after 30 h treatment compared with that of control group. These results showed the activities of defensive enzymes and contents of GSH in A. solani were decreased, whereas its conductivity and contents of MDA were increased by the T. asperellum fermentation broth, resulting in the inhibition of growth and development of A solani.

杨帅,孙丽丽,邓世林,袁碧玉,苏佳满,王志英,孙卉. 棘孢木霉发酵液对番茄早疫病抗氧化系统和脂质过氧化作用的影响[J]. 中国农学通报, 2017, 33(5): 90-94.

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棘孢木霉发酵液对番茄早疫病抗氧化系统和脂质过氧化作用的影响

Effects of Trichoderma asperellum Fermentation Broth on Antioxidant System and Lipid Peroxidation of Alternaria solani Sorauer

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