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中国农学通报

中国农学通报 ›› 2017, Vol. 33 ›› Issue (27): 43-48.doi: 10.11924/j.issn.1000-6850.casb16120122

所属专题： 资源与环境

• 生物技术科学 • 上一篇    下一篇

俄罗斯黄花草木樨生物活性成分资源的分布与开发利用

林年丰,梁 硕   

  1. 吉林大学环境与资源学院,JiLin University
  • 收稿日期:2016-12-27 修回日期:2017-09-04 接受日期:2017-02-24 出版日期:2017-10-09 发布日期:2017-10-09
  • 通讯作者: 林年丰
  • 基金资助:
    公益性行业（农业）科研专项经费项目“黄花草木樨—‘斯列金1 号’防治土壤退化与综合开发示范试验研究”(200903031)。

The Distribution, Development and Utilization of Bioactive Components Resources of Russian Melilotus officinalis

林年丰 and   

  • Received:2016-12-27 Revised:2017-09-04 Accepted:2017-02-24 Online:2017-10-09 Published:2017-10-09

摘要： 为了查明生物活性成分在黄花草木樨根、茎、叶、子中的含量与分布特征,本研究重点开展了黄花草木樨活性成分的检测及田间开发试验。分别采用凯式定氮法和高效气相色谱法测蛋白质；氨基酸自动分析仪法测氨基酸；气相色谱-质谱、分子蒸馏仪检测脂肪酸；重量法检测皂苷与生物碱；高效液相色谱仪检测香豆素；福林试剂比色法检测多酚；蒽铜-硫酸比色法检测多糖；亚硝酸钠-硝酸铝-氢氧化钠比色法检测黄酮。按产量测算后,得出每公顷牧草生物活性成分的总产量为3016.3 kg/hm2。其中粗蛋白质、氨基酸和粗脂肪酸原料的产量分别为1400.6 kg/hm2,112.2 kg/hm2,1246.8 kg/hm2。有效成分皂苷、生物碱、多酚、香豆素的产量分别为97.0 kg/hm2、77.9 kg/hm2、41.7 kg/hm2、40.1 kg/hm2。上述成分经分离提取纯化后,可作为开发医药、食物、化工等生物技术产品的天然原料。本文为开发利用植物自然资源提供了新的途径。

关键词: 地下渗滤, 地下渗滤, 脱氮效果, 影响因素, 强化措施

Abstract: [Objective] We have focused on the detection and field development test of Melilotus officinalis bioactive ingredients since 2009 in order to find outSthe content and distribution of bioactive ingredients in Russian Melilotus officinalis. [Methods]We use Kjeldahl method and efficient gas chromatography to measure the proteinScontent, automaticSaminoSacidSanalyzer to measure theSkinds and contents of aminoSacid, Gas chromatography - mass spectrometry (GC - MS) and molecular distillation apparatus to measure the fatty acid content, gravimetric method to measure the Saponin and alkaloid contents, high performance liquid chromatograph to measure the coumarin content, the forint reagent colorimetric method to measure the polyphenol content, anthracene copper - sulfuric acid colorimetry to measure the polysaccharide content, and aluminum nitrate-sodium nitrite-sodium hydroxide colorimetric method to measure the flavone content.[Result]This paper shows the bioactive ingredients content and distribution featuresSin Melilotus officinalis’ roots, stems, leaves, seeds, and measures the total production of bioactive ingredients, i.e. 3016.3 kg/hm2. The bioactive ingredients, crude protein, amino acids and fatty acids, are 1400.6 kg/hm2, 112.2 kg/hm2, 1246.8 kg/hm2, respectively. The output of saponins, alkaloids, polyphenols, coumarin are 97.0 kg/hm2, 77.9 kg/hm2, 41.7 kg/hm2, 40.1 kg/hm2,respectively. [Conclusion] After separation, extraction and purification, the aboveScomponents can be used as the natural ingredients to develop the medicine, food, chemical and other biotechnology products. This paper provides a new way for the development and utilization of natural resources of plants.

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