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中国农学通报

中国农学通报 ›› 2019, Vol. 35 ›› Issue (28): 32-37.doi: 10.11924/j.issn.1000-6850.casb18050085

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

紫苏细胞悬浮培养生产迷迭香酸的条件优化

李会珍, 李 娜, 谭永兰, 张志军   

  1. 中北大学化学工程与技术学院
  • 收稿日期:2018-05-15 修回日期:2018-07-20 接受日期:2018-07-25 出版日期:2019-10-14 发布日期:2019-10-14
  • 通讯作者: 张志军
  • 基金资助:
    山西省专利推广实施资助专项“从紫苏叶中提取迷迭香酸的方法”(20171008)；山西省重点研发项目“特色油用植物紫苏的产业技术开发 与示范”(201703D211005)。

Optimization of Suspension Culture Condition for Producing Rosmarinic Acid from Perilla Cells

  • Received:2018-05-15 Revised:2018-07-20 Accepted:2018-07-25 Online:2019-10-14 Published:2019-10-14

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摘要： [目的]本研究以紫苏愈伤组织为原材料,对液体悬浮培养生产迷迭香酸的培养条件进行了研究。[方法]通过单因素试验,分别考察培养时间、初始接种量、激素浓度及苯丙氨酸浓度对迷迭香酸含量的影响,并在单因素试验基础上,采用Box-Behnken中心组合设计和响应面法对最佳培养条件进行优化,建立了二次多项式回归方程的预测模型。[结果][结论]研究结果表明,紫苏细胞液体悬浮培养生产迷迭香酸的最佳培养条件为：6-BA浓度2.34 mg/L、NAA浓度0.96 mg/L、2,4-D浓度0.60 mg/L、苯丙氨酸浓度0.1 g/L,迷迭香酸含量可达23.61 mg/g。

关键词: 绿豆, 绿豆, 农艺性状, 因子分析, 综合评价

Abstract: [Objective]With perilla (Perilla frutescens) callus as raw material, the cell suspension culture conditions for the production of rosmarinic acid were studied. [Method]The effects of culture time, initial inoculum amount, hormone concentration and phenylalanine concentration on the content of rosmarinic acid were investigated by single-factor tests. The optimal culture conditions were optimized by Box-Behnken Center design of response surface method, and a quadratic polynomial regression equation of the forecasting model was established. [Result][Conclusion]The results showed that the optimal conditions for the production of rosmarinic acid by suspension culture of perilla cells were as follows: 6-BA of 2.34 mg/L, NAA of 0.96 mg/L, 2, 4-D of 0.60 mg/L, phenylalanine of 0.1 g/L. Under these conditions, the rosmarinic acid content in perilla callus was up to 23.61 mg/g.

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