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中国农学通报

中国农学通报 ›› 2023, Vol. 39 ›› Issue (24): 84-89.doi: 10.11924/j.issn.1000-6850.casb2022-0726

• 资源·环境·生态·土壤 • 上一篇    下一篇

金钱草—水稻轮作对土壤微生物群落结构的影响

练瀚1(), 韩蓉蓉2, 杨小玉2, 唐祥友2, 张兴翠1, 胡开治2()   

  1. 1 西南大学农学与生物科技学院，重庆 400700
    2 重庆市药物种植研究所，重庆 408435
  • 收稿日期:2022-08-22 修回日期:2022-12-02 出版日期:2023-08-25 发布日期:2023-08-23
  • 通讯作者: 胡开治，男，1968年出生，重庆南川人，研究员，硕士，研究方向：中药材品种选育与栽培技术研究。通信地址：408435 重庆市南川区三泉镇，Tel：023-71480053，E-mail：402632144@qq.com。
  • 作者简介:

    练瀚，男，1995年出生，贵州遵义人，硕士研究生，研究方向：药用植物栽培与育种。通信地址：400715 重庆市北碚区天生街道，E-mail:。

  • 基金资助:
    重庆市技术创新与应用发展专项重点项目“基于农艺学、中药化学、药效学系统筛选金钱草良种与应用”(cstc2019jscx-gksbx0010); 国家重点研发计划项目“林下生态种植黄连、灵芝等中药材产业关键技术研究与应用示范”(2021YFD1601005)

Effects of Lysimachia christinae Hance—Rice Rotation on Soil Microbial Community Structure

LIAN Han1(), HAN Rongrong2, YANG Xiaoyu2, TANG Xiangyou2, ZHANG Xingcui1, HU Kaizhi2()   

  1. 1 College of Agronomy and Biotechnology, Southwest University, Chongqing 400700
    2 Chongqing Institute of Medicinal Plant Cultivation, Chongqing 408435
  • Received:2022-08-22 Revised:2022-12-02 Online:2023-08-25 Published:2023-08-23

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摘要：

通过分析金钱草—水稻水旱轮作模式对金钱草土壤微生物群落的影响，为中药金钱草的高效、高产栽培和持续种植提供理论依据。采用高通量测序技术，分析金钱草—水稻轮作对土壤微生物群落的组成和多样性差异。结果表明：土壤细菌OTU数量在金钱草水稻轮作前后无显著差异，真菌OUT数量显著减少；细菌的多样性和丰富度在轮作前后无显著性变化，真菌多样性无显著性变化，但丰富度显著降低。在门水平上，供试土壤共检测出22个细菌门，包括变形菌门、酸杆菌门、放线菌门等，共检测出8个真菌门，主要有子囊菌门、担子菌门、被孢霉门和罗泽洛菌门等；除酸杆菌门相对丰富度在水稻轮作后显著增加外，其他细菌门和真菌门相对丰富度都呈现减少趋势。金钱草-水稻轮种能减少土壤微生物相对丰富度，特别是真菌的相对丰富度显著减少，作物连作过程会使土壤微生物动态演替表现出真菌数量增加的趋势，以期为进一步研究金钱草连续种植下的连作障碍和降低病害研究提供一定理论依据。

关键词: 金钱草, 水旱轮作, 高通量测序, 土壤微生物

Abstract:

The analysis of changes of microbial community in the soil under the rotation of Lysimachia christinae Hance—rice was carried out to provide a theoretical basis for the efficient, high-yield and sustainable cultivation of Lysimachia christinae Hance. High-throughput sequencing technology was used to analyze the composition and diversity of soil microbial communities under the rotation of Lysimachia christinae Hance—rice. The results showed that there was no significant difference in the number of soil bacterial OTU under the rotation of Lysimachia christinae Hance—rice, while the number of soil fungal OUT decreased significantly. The diversity of bacteria, same as fungi, did not change significantly before and after the rotation. The richness of bacteria reduced significantly, but the richness of fungi did not change significantly. At the phylum level, a total of 22 bacterial phyla were detected in the soil including Proteobacteria, Acidobacteria, Actinomycetes, etc., and a total of 8 fungal phyla were detected including Ascomycota, Basidiomycota, and Cystobacteria, etc.. Except that the relative abundance of Acidobacteriaceae increased significantly after rice rotation, the relative abundances of other bacterial phyla and fungal phyla showed a decreasing trend. The Lysimachia christinae Hance—rice rotation could reduce the relative richness of soil microorganisms, especially the relative richness of fungi. The continuous cropping process would make the dynamic succession of soil microorganisms show a trend of increasing the number of fungi. This research could provide a theoretical basis on continuous cropping obstacles and disease reduction under continuous grass planting of Lysimachia christinae Hance.

Key words: Lysimachia christinae Hance, dry and dry crop rotation, high-throughput sequencing, soil microorganisms