单细胞转录组在昆虫学研究的应用

doi:10.11924/j.issn.1000-6850.casb2023-0105

中国农学通报 ›› 2023, Vol. 39 ›› Issue (17): 79-85.doi: 10.11924/j.issn.1000-6850.casb2023-0105

单细胞转录组在昆虫学研究的应用

李媛媛(), 德力格尔, 刘晶, 石凯()

内蒙古民族大学农学院，内蒙古通辽 028043

收稿日期:2023-02-02 修回日期:2023-04-05 出版日期:2023-06-15 发布日期:2023-06-12
通讯作者: 石凯，女，教授，博士，研究方向：草地昆虫与害虫防治。通信地址：028043 内蒙古通辽市科尔沁区霍林河大街西536号内蒙古民族大学农学院，E-mail：nmshikai@126.com。
作者简介:
李媛媛，女，1982年出生，河北唐山人，副教授，硕士，研究方向：草地昆虫与害虫防治。通信地址：028043 内蒙古通辽市科尔沁区霍林河大街西536号内蒙古民族大学农学院，E-mail：liyuanyuan20131128@126.com。
基金资助:
内蒙古自治区自然科学基金项目“内蒙古东部农牧交错带盲蝽科多样性分化、成因与年际差异”(2022LHMS03016); 内蒙古自治区科技计划项目“蒙东草原典型露天矿区生态修复技术集成及示范”(2022YFDZ0073); 内蒙古自治区高等学校科学研究项目“北方草原草盲蝽复合组昆虫多样性与地理分布研究”(NJZY18163)

Advances in Insect Single-Cell Transcriptomics

LI Yuanyuan(), DELIGEER, LIU Jing, SHI Kai()

College of Agriculture, Inner Mongolia Minzu University, Tongliao, Inner Mongolia 028043

Received:2023-02-02 Revised:2023-04-05 Online:2023-06-15 Published:2023-06-12

摘要/Abstract

摘要：

为了解析昆虫表型背后的基因调控机制，单细胞转录组测序克服了传统技术的缺陷，充分体现了细胞异质性，实现了细胞类型的鉴定、细胞的分化轨迹的推导、细胞间互作关系的分析。总结了单细胞转录组在昆虫学研究方面的应用，以期为昆虫表型—细胞—基因的功能研究和分子机制研究提供参考借鉴。

关键词: 昆虫, 单细胞转录组, 混合细胞转录组, 转录组学, 研究进展

Abstract:

To analyze the gene regulatory mechanisms behind insect phenotypes, single-cell transcriptome sequencing technology overcomes the shortcomings of traditional techniques and fully reflects cellular heterogeneity, and realizes the identification of cell types, the derivation of cell differentiation trajectories, and the analysis of intercellular interactions. This paper summarized the application of single-cell transcriptomes in entomological studies, with a view to providing references for insect phenotype-cell-gene studies in function and molecular mechanisms.

Key words: insects, single-cell transcriptome, bulk-cell transcriptome, transcriptomics, research progress

李媛媛, 德力格尔, 刘晶, 石凯. 单细胞转录组在昆虫学研究的应用[J]. 中国农学通报, 2023, 39(17): 79-85.

LI Yuanyuan, DELIGEER, LIU Jing, SHI Kai. Advances in Insect Single-Cell Transcriptomics[J]. Chinese Agricultural Science Bulletin, 2023, 39(17): 79-85.

图/表 1

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物种	组织	虫态	细胞数量	测序平台	参考文献
果蝇	脑	1龄幼虫	9363	10x Genomics	[59]
果蝇	脑	雌雄成虫	56902	10x Genomics	[60]
果蝇	中肠	7日龄雌性成虫	10605	10x Genomics	[62]
果蝇	卵巢	3龄幼虫	3424	10x Genomics	[66]
冈比亚按蚊	血淋巴	雌性成虫	262	SMART-seq2 methodology	[65]
家蚕	丝腺	胚胎、蚁蚕和1龄眠期	14972	10× Genomics	[67]
法老蚁	脑	工蚁、处女繁殖蚁、蚁后、雄蚁	206367	DNBSEQ	[61]

物种	组织	虫态	细胞数量	测序平台	参考文献
果蝇	脑	1龄幼虫	9363	10x Genomics	[59]
果蝇	脑	雌雄成虫	56902	10x Genomics	[60]
果蝇	中肠	7日龄雌性成虫	10605	10x Genomics	[62]
果蝇	卵巢	3龄幼虫	3424	10x Genomics	[66]
冈比亚按蚊	血淋巴	雌性成虫	262	SMART-seq2 methodology	[65]
家蚕	丝腺	胚胎、蚁蚕和1龄眠期	14972	10× Genomics	[67]
法老蚁	脑	工蚁、处女繁殖蚁、蚁后、雄蚁	206367	DNBSEQ	[61]

单细胞转录组在昆虫学研究的应用

Advances in Insect Single-Cell Transcriptomics

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