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中国农学通报 ›› 2011, Vol. 27 ›› Issue (13): 249-252.

所属专题: 园艺

• 林学 园艺 园林 • 上一篇    下一篇

防治草莓连作障碍的复合生防制剂的筛选

扈金丽 尹宝重 甄文超 刘盼   

  • 收稿日期:2011-01-27 修回日期:2011-03-18 出版日期:2011-06-05 发布日期:2011-06-05
  • 基金资助:

    基于微生态调控的设施草莓连作障碍综合防治技术体系的研究与应用

Selection of Complex Biological Control Agents to Prevent Replant Diseases on Strawberry

  • Received:2011-01-27 Revised:2011-03-18 Online:2011-06-05 Published:2011-06-05

摘要:

为有效解决单一使用解酚菌或药用植物材料防治草莓连作障碍过程靶标单一的问题,拟将二者有效结合制成复合生防制剂,达到祛毒抑菌的作用。本试验选用解酚菌B3512与6种对草莓枯萎病有抑制作用的药用植物(姜黄、青皮、山柰、枳实、Z1、Z2)为试材,测定B3512在6种药用植物材料上的生长速率。结合抑菌试验,确定以姜黄为基质,玉米秸秆粉、麦麸为碳源、氮源时,B3512的生长速率最快且对草莓枯萎病病原菌的抑菌率最高;同时筛选出以姜黄为基质利于B3512定植,增殖的固体发酵培养基配方及条件,配方为麦麸:玉米秸秆粉:姜黄按20:10:6的比例混合,B3512以20%的接种量、最适温度32℃、初始含水量50%、初始pH 7.0、培养96 h时,B3512的繁殖能力最强且活菌数最高。

关键词: 玉米增效剂, 玉米增效剂, 玉米杂交种, 农艺性状

Abstract:

In order to solve the problem of using phenol solution bacteria or medicinal to control strawberry plant, proposed to effectively combine the two into complex biological control agents to Qudu inhibitory effect. This study used phenolic acid degradation fungi B3512 and six kinds of medicinal plant material (turmeric, peel, kaempferol, citrus aurantium, Z1, Z2) which had inhibited to strawberry wilt strongly as test materials, through measured the growth rate of B3512 in six different medicinal plants and with antimicrobial test. Finally we identified that B3512 had the fastest growth rate and the highest inhibition rate of strawberry wilt pathogen when turmeric was considered as substrate, corn straw flour and wheat bran as carbon and nitrogen source. At the same time we selected the solid fermentation medium and conditions which benefited strain B3512 colonization and proliferation. The last formula was wheat bran: corn straw powder: turmeric at the ratio of 20:10:6. And when the inoculum size of phenolic acid degradation fungi B3512 was 20%, optimum temperature was 32℃, initial moisture content was 50%, initial pH was 7.0, B3512 had the highest reproductive capacity and the highest number of viable cells.

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