单细胞转录组测序技术及其在植物研究中的应用

doi:10.11924/j.issn.1000-6850.casb2021-0316

中国农学通报 ›› 2022, Vol. 38 ›› Issue (3): 87-93.doi: 10.11924/j.issn.1000-6850.casb2021-0316

所属专题：生物技术

单细胞转录组测序技术及其在植物研究中的应用

陈柳宏¹^,²(), 赵春雷¹^,³, 王希¹^,³, 李彦丽¹^,³^,⁴, 丁广洲¹^,²(), 陈丽¹^,³()

¹黑龙江大学现代农业与生态环境学院,哈尔滨 150080
²黑龙江省甜菜工程技术研究中心,哈尔滨 150080
³国家糖料改良中心,哈尔滨 150080
⁴国家甜菜种质中期库,哈尔滨 150080

收稿日期:2021-03-26 修回日期:2021-07-08 出版日期:2022-01-25 发布日期:2022-02-25
通讯作者: 丁广洲,陈丽
作者简介:陈柳宏,女,1998年出生,四川达州人,在读硕士,研究方向：甜菜分子育种与种质资源,参与黑龙江省经济作物产业技术协同创新体系甜菜育种岗位科学家项目、国家糖料产业技术体系（甜菜品种资源评价）岗位科学家项目等。通信地址：150080 黑龙江大学现代农业与生态环境学院,Tel：18702256452,E-mail： 251272275@qq.com。
基金资助:
国家现代农业产业技术体系（糖料）建设专项资金资助”(Supported by Sugar Crop Research System, CARS)CARS-170102;黑龙江省经济作物产业技术协同创新体系项目“甜菜育种岗位”(2018711)

Single-cell Transcriptome Sequencing Technology and Its Application in Plant Research

CHEN Liuhong¹^,²(), ZHAO Chunlei¹^,³, WANG Xi¹^,³, LI Yanli¹^,³^,⁴, DING Guangzhou¹^,²(), CHEN Li¹^,³()

¹College of Advanced Agriculture and Ecological Environment, Harbin 150080
²Sugarbeet Engineering Research Centre of Heilongjiang Province, Harbin 150080
³National Sugar Crops Improvement Center, Harbin 150080
⁴National Beet Germplasm Medium Term Storeroom, Harbin 150080

Received:2021-03-26 Revised:2021-07-08 Online:2022-01-25 Published:2022-02-25
Contact: DING Guangzhou,CHEN Li

摘要/Abstract

摘要：

为探究单细胞转录组测序技术(scRNA-seq)在植物研究中的应用,本综述对单细胞转录组测序技术的发展状况进行了总结,介绍了其在胚胎发育、免疫细胞、肿瘤领域以及植物研究的运用。其次,就单细胞转录组测序技术中的要点技术（单细胞分离技术、单细胞全转录组cDNA扩增技术、高通量测序技术）进行了总结分析。最后,本文根据植物细胞的特点归纳出植物所适用的单细胞转录组测序技术,以期为该技术在植物研究中更好的利用提供理论依据。

关键词: 植物, 单细胞转录组测序, 转录组学, 单细胞分离技术, 高通量测序

Abstract:

In order to explore single-cell transcriptome sequencing technology (scRNA-seq) application in plant research, this review summarized the development status of scRNA-seq, and introduced its applications in embryonic development, immune cells, tumors and plant research. Secondly, this article analyzed the key technologies of scRNA-seq (ie. single-cell separation, single-cell whole transcriptome cDNA amplification technology and high-throughput sequencing). Finally, this article summed up the scRNA-seq applicable to plants based on the characteristics of plant cells, aiming to provide a theoretical basis for the better use of the method in plant research.

Key words: plant, single-cell transcriptome sequencing, transcriptomics, single-cell separation technology, high-throughput sequencing

中图分类号:

陈柳宏, 赵春雷, 王希, 李彦丽, 丁广洲, 陈丽. 单细胞转录组测序技术及其在植物研究中的应用[J]. 中国农学通报, 2022, 38(3): 87-93.

CHEN Liuhong, ZHAO Chunlei, WANG Xi, LI Yanli, DING Guangzhou, CHEN Li. Single-cell Transcriptome Sequencing Technology and Its Application in Plant Research[J]. Chinese Agricultural Science Bulletin, 2022, 38(3): 87-93.

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单细胞转录组测序技术及其在植物研究中的应用

Single-cell Transcriptome Sequencing Technology and Its Application in Plant Research

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