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中国农学通报

中国农学通报 ›› 2007, Vol. 23 ›› Issue (9): 3-3.

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

矮生香石竹的组织培养和快速繁殖

黄冬华,周 群,陶秀花,宋小民,李宝光,倪国平   

  • 出版日期:2007-09-05 发布日期:2007-09-05

Tissue Culture and Rapid Propagation of the Short Carnation

Huang Donghua,Zhou Qun, Tao Xiuhua,Song Xiaomin, Li Baoguang, Ni guoping   

  • Online:2007-09-05 Published:2007-09-05

摘要： 矮生香石竹（代号为SJ-3）是本所采用切花香石竹和日本石竹杂交而成的优良新品种，该品种矮生、重瓣，没有种子，不能进行有性繁殖，因此利用组织培养技术、以矮生香石竹茎尖段为外植体，进行了组织培养快速繁殖研究。试验结果表明：（1）矮生香石竹增殖分化阶段的合适培养基为MS+6-BA0.2 mg/L +KT 0.1 mg/L+NAA 0.05 mg/L+CCC 5 ml/L；（2）合适的生根培养基为1/2MS+NAA0.5 mg/L+IBA0.5 mg/L，生根率在86.6%；（3）在同等条件下采用透气的封口膜对克服矮生香石竹试管苗玻璃化有明显的效果；（4）生根试管苗移入珍珠岩∶河沙∶泥炭＝1∶1∶1的基质中，成活率达93%，同时采用瓶外生根技术移栽成活率高，并缩短了试管苗的繁殖周期。

关键词: 保水剂, 保水剂, 吸水倍率, 腐殖酸钠, 缓释

Abstract: The short Carnation (code number is SJ-3) is a fine variety witch was cultivated by hybridization of the Carnation and the Japanese dianthus. The variety has some characters of dwarf, double petals, no seed and cannot carry out sex-reproduction. Accordingly, the speed propagation experiment was conducted to study by tissue culture technology.The tissue culture of stem point section from short Carnation was studied. The results showed:(1)The suitable medium for induction culture and multiplication of the short Carnation was MS+6-BA 0.2mg/L+KT0.1mg/L+NAA0.05mg/L+CCC5ml/L;(2)The suitable rooting medium was improved MS+ NAA0.5 mg/L +IBA0.5 mg/L, with rooting rate 86.6%.(3)Under the same level condition, the effect to overcome the short Carnation ventilation was obvious when uses the seal membrane. (4)The root－developed plantlets transplanted in the substrate mixed with perlite sand and peat (volume ratio of 1:1:1),the survival rate reaches above 93%.The survival rate of transplant was the highest by rooting outside the bottle and abbreviated the propagation periods of test tube seedling.

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