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中国农学通报 ›› 2008, Vol. 24 ›› Issue (9): 72-77.

• 生物技术科学 • 上一篇    下一篇

EST分子标记开发研究进展

陈全求,詹先进,蓝家样,黄 云   

  • 收稿日期:2008-05-01 修回日期:2008-05-29 出版日期:2008-09-05 发布日期:2008-09-05

Study Progress in Development of EST (expressed sequence tags)

Chen Quanqiu, Zhan Xianjin, Lan Jiayang, Huang Yun   

  • Received:2008-05-01 Revised:2008-05-29 Online:2008-09-05 Published:2008-09-05

摘要: EST(expressed sequence tags)计划作为植物基因组计划的一个重要组成部分,已经在多种植物物种中开展起来,EST资源库的不断扩充为利用这些数据来开发EST分子标记奠定了基础。概括介绍了几种主要EST标记(EST-SSR,EST-PCR,EST-SNP,EST-AFLP,EST-RFLP)的开发策略,同时也提出了EST标记开发存在的一些问题及解决方法。

关键词: 晋南, 晋南, 日光温室, 微生物, 土壤酶

Abstract: The plant EST (expressed sequence tags) program is one of the most important parts which compose the plant genome program. The expansion of EST data base is foundation of development of EST markers. In this paper, the development of five EST markers (EST-SSR, EST-PCR, EST-SNP, EST-AFLP, and EST-RFLP). Some problem exit in the development of EST markers is also discussed.

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