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中国农学通报 ›› 2010, Vol. 26 ›› Issue (3): 206-215.

所属专题: 资源与环境

• 林学 园艺 园林 • 上一篇    下一篇

澳洲坚果种质资源形态性状的遗传多样性分析

贺熙勇,倪书邦,陈国云,魏丽萍,肖晓明,陶丽   

  • 收稿日期:2009-10-09 修回日期:2009-11-01 出版日期:2010-02-05 发布日期:2010-02-05
  • 基金资助:

    澳洲坚果良种筛选及配套栽培技术试验示范;澳洲坚果种质资源的收集、保存与利用研究

Morphological Characteristics Analysis on Genetic Diversity of Macadamia (Macadamia spp.) Germplasm Resources

  • Received:2009-10-09 Revised:2009-11-01 Online:2010-02-05 Published:2010-02-05

摘要:

为了解引入澳洲坚果种质的遗传背景,以64份澳洲坚果种质为试材,采用41个形态性状对其进行了遗传多样性分析。41个形态性状的差异性和R型聚类分析结果表明:各性状间差异达到极显著水平;在欧氏距离(D)≈11.72处,41个性状聚为两组,组内各性状的欧氏距离较远,绝大多数性状间相对独立,对澳洲坚果种质的演化各具独立的意义,在澳洲坚果的分类中有较好的分辨能力。Q型聚类分析结果表明:64份种质的欧氏距离在3.15~12.39之间,具有较高的遗传多样性。在D≈12处可将64份种质划分为二个类群:第一类群仅包含T2 1份种质,为澳洲坚果栽培种的近缘种;第二类群包含剩下的63份种质,为澳洲坚果的栽培种。第二类群在D≈10处,可细分成3个亚类:第Ⅰ亚类包括7份种质(即695、Tet.-2、900、D4、HY、Tet.-1和广9),属于粗壳种与光壳种的杂交种;第Ⅱ亚类包括A4和A16两个品种,属于粗壳种与光壳种的杂交种,但更偏重于光壳种;第Ⅲ亚类包括其他54份种质,都是光壳种的后代。获得的聚类结果与现有的分类体系一致;同时,获得了64份种质的形态性状聚类的亲缘关系图,其在生产种植中的品种搭配、育种中的杂交亲本选择和引种方面都具有指导意义。

关键词: 小区绿化, 小区绿化, 植物, 造景

Abstract:

In order to know genetic background of macadamia germplasms introduced, a genetic diversity analysis involving 64 accessions of Macadamia was performed using 41 morphological characteristics. Statistic analysis and R-style cluster analysis were used to examine the 41 morphological characteristics (including 19 quantitative traits and 22 qualitative traits) of macadamia based on the software DPSv7.05. The result showed: there were striking dissimilarity among characteristics; at the Euclidean distance approximately 11.72, the 41 characteristics were divided into two groups. There were significant differences among characteristics within their group. Most characteristics were independent one another and had important meaning in macadamia’s taxology. The result, based on Q-style cluster analysis, showed: Euclidean distance of 64 germplams ranging from 3.15 to 12.39, indicated high level of genetic diversity in these germplams. And the 64 germplams were divided into two groups at the Euclidean distance approximately 12. Group one included only T2,which belonged to related species of macadamia cultivation. Group two included the rest, which could be further divided into three sub-groups at the Euclidean distance approximately 10. The first sub-group included seven accessions: 695, 900, D4, HY, Guang9, Tet.-1, and Tet.-2, which belonged to hybrids of M.integrifolia and M. tetraphylla. The second sub-group included two accessions: A4 and A16, which also belongs to hybrids of M.integrifolia and M. tetraphylla, but were more similar to M. integrifolia. The third sub-group included the other 54 accessions that were all offsprings of M integrifolia. The cluster result, based on morphological characteristics analysis, was consistent with the existing system of taxology. Meanwhile, the blood relationship figure of 64 accessions was also obtained, and could be applied to combinations of cultivars, selection of hybrid parent and introduction from abroad.