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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (19): 22-26.

所属专题： 生物技术； 小麦

• 生物技术科学 • 上一篇    下一篇

小麦基因组的一种简易提取方法

师丽红 杨文香 刘大群   

  • 收稿日期:2010-04-28 修回日期:2010-05-24 出版日期:2010-10-05 发布日期:2010-10-05
  • 基金资助:

    国家公益性行业（农业）科研专项，国家“十一五”支撑计划

One simple method for extracting of the wheat genome

  • Received:2010-04-28 Revised:2010-05-24 Online:2010-10-05 Published:2010-10-05

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摘要：

摘要：【研究目的】在综合分析多种提取小麦DNA方法的基础上，改良发展了一种快速简便高效的小麦基因组DNA提取方法。【方法】以春小麦Thatcher和以Thatcher为背景的近等基因系TcLr19，TcLr20， TcLr28为材料，改进后的方法提取的基因组DNA用抗叶锈基因Lr20的STS标记、Lr19和Lr28的SCAR标记、Lr28的SSR 标记进行PCR扩增，【结果】各分子标记都扩增出条带清晰正确的目的片段【结论】这表明该方法能够获得适用于STS、SCAR、SSR标记PCR扩增的高质量的DNA，而且该DNA简易提取方法加快了DNA提取速度、降低了污染源和成本，为大批量提取DNA提供了技术支撑。

关键词: 福建, 福建, 农业竞争力, 综合评价, 动态分析

Abstract:

Abstract:【Objective】 In a comprehensive analysis of a variety of wheat genomic DNA extraction methods, one easy and efficient wheat genomic DNA extraction method had been improved and developed.【Method】 Spring wheat cultivar Thatcher, TcLr19, TcLr20 and TcLr28 as the materials were used to extract the DNA. And then was used as template to amplify the STS marker for leaf rust resistance gene Lr20, SCAR marker for Lr19 and Lr28, SSR marker for Lr28. 【Result】The result showed that clear and right bands or smear were amplified. 【Conclusion】It means that the method could get the high quality DNA for PCR analysis. This method reduce the DNA extracting processing, costs, and pollution to the environment and human being, and therefore provided solid base for the preparation of large number of samples for PCR-based assays.