欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (1): 415-418.

所属专题： 生物技术； 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

鸭肝炎病毒单克隆抗体的制备

潘朋朋 文力正 单振振 丁毅 袁宝 任文陟 李维娜 李晓叶   

  • 收稿日期:2010-06-25 修回日期:2010-08-02 出版日期:2011-01-07 发布日期:2011-01-07
  • 基金资助:

    吉林省实验动物质量检测中心平台建设项目（20071138）

Preparation of Monoclonal Antibody against Duck Hepatitis Virus

  • Received:2010-06-25 Revised:2010-08-02 Online:2011-01-07 Published:2011-01-07

PDF (PC)

882

摘要：

本实验利用弗式完全佐剂和弗式不完全佐剂，乳化病毒，制备免疫抗原，对BALB/c小鼠进行三次免疫。将免疫效价达到1：10000以上的免疫小鼠脾细胞与SP2/0细胞进行融合，通过间接ELISA方法对阳性杂交瘤细胞株进行筛选，并通过有限稀释法将呈强阳性的杂交瘤细胞亚克隆3～4次，直到杂交瘤细胞阳性率达到100%。得到阳性株命名为2E3，对阳性株细胞进行扩大培养，并将细胞注入腹腔，提取腹水，测定细胞上清及腹水的效价,分别为10×25，10×26。利用亚类鉴定试剂盒测定单抗的亚类。鉴定结果为IgG1型。

关键词: 生物表面活性剂, 生物表面活性剂, 生物修复, 石油污染, 土壤, 微生物

Abstract:

In order to make DHV antigens,the experiment utilize IFA and CFA. The BALB/c mice were immunized with DHV for three times . The spleen’s B lymphocyte of the mice should be fused with SP2/0 plasmacytoma cells until the immunopotency was up to 1:10000.The masccline hybridoma cells were screened by indirect enzyme-link immunosorbent assay. Hybridomas whose supernatants show stronger positive were subcloned by limited dilution methods for 3~4 times, until the positive rate come up to 100%.It was named 2E3. The immunopotency of ascites and supernatant was 10×25,100×26..And the subtype of 2E3 was IgGl.

中图分类号: