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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (7): 290-294.

所属专题： 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

猪圆环病毒2型间接ELISA方法的建立

车勇良 庄向生 石运通 王隆柏 魏宏 陈如敬 吴学敏 周伦江   

  • 收稿日期:2010-10-09 修回日期:2010-11-23 出版日期:2011-04-05 发布日期:2011-04-05
  • 基金资助:

    福建省科技计划重点项目

Establishment of indirect ELISA method to detect Porcine Circovirus 2 antibody

  • Received:2010-10-09 Revised:2010-11-23 Online:2011-04-05 Published:2011-04-05

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摘要：

为了猪圆环病毒2型（PCV2）抗体，本研究建立了快速、敏感的ELISA诊断方法。本研究应用原核表达的PCV2-Cap蛋白作为包被抗原，通过抗原最适包被浓度和兔抗猪IgG最佳稀释浓度的确定，待检血清稀释度的选择，阴阳性临界值的确定，然后进行特异性测定、重复性测定和符合率比较等，建立检测PCV2抗体的间接ELISA方法。结果显示，建立的间接ELISA方法只能检测出PCV2阳性血清；3次重复性试验的差异不显著；与韩国JBT公司的PCV2试剂盒的符合率达到了92.5%；对采集福建省内多个猪场204份血清样品进行检测，其阳性率达到了76.5%。实验表明，建立的检测PCV2抗体的间接ELISA方法具有特异性强、重复性和稳定性好的特点，可用于PCV2抗体的血清学诊断和流行病学调查。

关键词: 农艺性状, 农艺性状

Abstract:

The research established a fast and sensitive indirect ELISA method to detect PCV2 antibody. The experiment used the prokaryotic expressive PCV2-Cap protein as the envelope antigen. In addition, we also confirmed the optimal coating-antigen concentration、Rabbit Anti-Pig IgG concentration, and selected the best detection-pending serum dilution、critical value, and detected specificness、stability、coincidence rate by indirect ELISA method. The results indicated only the PCV2 postive serum was detected by indirect ELISA; The variance among the three repeat experiments was nonsignificant; The coincidence rate between indirect and the PCV2 Kit of JBT in Korea was up to 92.5%; The 204 swine serum samples from the Fujian Province were detected by indirect ELISA, the positive rate was 76.5%. The experiment revealed the optimization indirect ELISA was of good specificity, good repeatability and stability, and may be used as the serodiagnosis and the epidemiological study of PCV2 antibody.

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