Abstract: The purpose was to select glutinous sorghum with high combining ability and heritability, and to improve crossbreeding efficiency, to provide a theoretic basis for breeding good parents and making up strong and excellent combinations during hybrid glutinous sorghum breeding. The 9 crossing combinations were made by three sterility lines and three restoring lines with the method of non-complete diallel crossing model. Combining ability and the genetic effects of 6 characters on plant height, ear length, weight per spike, growth period, 1000 grain weight, production were analyzed by DPS data statistics. The results showed that, in the three sterility lines, 11476A2 exhibited the highest general combining ability in plant height, ear length and growth period. With the relative volume of effectiveness were 2.5572, 2.4284, 0.6792. 11494A2 exhibited the highest general combining ability in weight per spike, production. With the relative volume of effectiveness were 5.7165, 4.8505. They had higher application potential in breeding and production. The order of ratio of additive genetic variance to genotype variance was: plant height > weight per spike > 1000 grain weight > production > ear length > growth period. As to yield traits, 11476A2×L17 was better heterotic pattern.