Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2022, Vol. 38 ›› Issue (34): 33-38.doi: 10.11924/j.issn.1000-6850.casb2022-0552

Special Issue: 生物技术 园艺

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Preliminary Study on the Bag Material Cultivation Techniques of Poria cocos

WANG Yinan1(), WANG Jibin2, ZHOU Fei1, WANG Wenzhi3, ZHANG Jinping1(), FANG Hong4   

  1. 1Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400
    2Chun’an County Forestry Bureau of Hangzhou, Hangzhou 311700
    3Anhui Tianfu Biotechnology Co., Ltd., Anqing, Anhui 246600
    4Chun'an County Forestry Station, Hangzhou 311700
  • Received:2022-07-07 Revised:2022-08-26 Online:2022-12-05 Published:2022-11-25
  • Contact: ZHANG Jinping E-mail:wyn510468915@163.com;jinpingzhang@126.com

Abstract:

This study takes pine branches and pine needles as raw materials, and studies the effects of different bag material formulas and cultivation methods on the sclerotium formation rate, biological efficiency and content of chemical components of Poria cocos, and compares the effects with those of the traditional log cultivation of P. cocos (control group), in order to provide technical support for the bag material cultivation of P. cocos. Two P. cocos bag material cultivation formulas obtained in the previous experiments were used to make the bag materials and the sclerotium of P. cocos was inoculated by the wood guide method. The biological efficiency and chemical components’ contents of P. cocos in single cellar were used as the comparative indexes to explore the effects of the two bag material formulas and different cultivation methods on the sclerotium formation and quality of P. cocos. The results showed that: the survival rate of P. cocos inoculated with the two formulas was 100%, and the highest biological efficiency of formula 1 was 38.18% higher than that of formula 2. The contents of alcohol soluble substances, total triterpenes and tuckaholic acid of formula 1 were 1.11%, 0.45% and 1569.81 mg/kg higher than those of formula 2, and those figures of formula 2 were 3.41%, 0.59% and 2053.98 mg/kg higher than those of P. cocos cultivated in log, respectively. The types and contents of amino acids (except cystine) in P. cocos of the two bag material cultivation formulas were 1.000-2.500 times those of P. cocos cultivated in log. The average biological efficiency and the highest biological efficiency of P. cocos inoculated in single bag were 6.09% and 10.17% higher than those in double bags.

Key words: Poria cocos, bag material formula, cultivation method, biological efficiency, chemical component

CLC Number: