Genetic Diversity Analysis of Major Male Sterile and Maintenance Monogerm Sugar Beet Line by SSR

doi:10.11924/j.issn.1000-6850.casb15070116

Abstract

Abstract: In this study, 14 pairs of SSR molecule markers were used to investigate the relationship of 41 pairs of Major Male Sterile and Maintenance Monogerm Sugar Beet Line, in order to prepare the combination of the fine single embryo, avoid the duplication of gene composition and improve the efficiency of breeding. Results showed that, a total of 93 unambiguous bands were obtained, 76 of which were polymorphic. On average, each pair of primer amplified 5.4 genotypes. The average ratio of polymorphic bands was 81.7%. Compute over-all mean showed that genetic distance was 0.3572, genetic similarity among varieties was 0.6996. The genetic similarities within different groups of genotypes were, 0.7549 among mixed one, 0.7513 among LAN 45-46, 0.7493 among LAN 71-72, 0.7465 among LAN 19-20, and 0.7337 among LAN 85-86. The 82 genotypes were divided into two cluster groups based on cluster analysis (at intercept of 0.2). The genotypes from Major Male Sterile and Maintenance Monogerm Sugar Beet Line showed high level of genetic diversity. There was a definite difference among different Male Sterile and Maintenance Monogerm genetic background.

CLC Number:

S566.3

Wang Maoqian,Ma Longbiao,Wu Zedong and Wang Huazhong. Genetic Diversity Analysis of Major Male Sterile and Maintenance Monogerm Sugar Beet Line by SSR[J]. Chinese Agricultural Science Bulletin, 2015, 31(32): 62-67.

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