Abstract: In field planting and production of sugarbeet, nitrogen nutrient should be supplied in time. The distribution feature of SPAD value on different leaf positions and leaf orders of sugarbeet was analyzed using SPAD-502 chlorophyll meter so that the rapid diagnostic technique of sugarbeet nitrogen nutrient status could be built on the SPAD value. Both pot and field experiment with different sugarbeet genotypes were conducted in order to measure the leaf SPAD value of different development stages in different genotypes of sugarbeet. Then, the authors counted and analyzed the correlations between the leaves’ SPAD value and its chlorophyll content or the plant nitrogen content in different genotypes of sugarbeet. The results were as follows: the leaves’ SPAD values of sugarbeet were different among different leaf positions and leaf orders. The repeated times of the biggest SPAD value of leaf tip were obviously more than that of the margin and lower part of the leaf, both treatments (N₁₅₀ and N₀) were all mixed up to 50%. The SPAD values of N₁₅₀ were higher than those of N₀, even they varied among different cultivars under each treatment. After 4-6 euphylla period of sugarbeet, the SPAD value of the highest leaf had a significant correlation with that of other leaves in the same plant, so the highest leaf tip of sugarbeet should be taken as the measured position of SPAD value. With high nitrogen application rates, the SPAD value increased. The SPAD values of different cultivars varied under each treatment. The linear equations were built respectively between SPAD values and chlorophyll concentration and plant total nitrogen content. After fitting the linear equations, the authors found that the SPAD value of leaf and total chlorophyll content of plant in different developmental stages (seedling, cluster rapid growth period and root sugar accumulation period) had a very significant correlation, the relationship between SPAD value of leaf and nitrogen content of plant was significant. So the development and photosynthetic efficiency of sugarbeet could be constantly monitored by SPAD value, and SPAD values of sugarbeet were suitable for rapid diagnosis of nitrogen nutrition.