Enhancing Effect of Alternative Oxidase on Sensitivity of Sclerotinia sclerotiorum to Carbendazim

doi:10.11924/j.issn.1000-6850.casb15090138

Abstract

Abstract: In order to study whether alternative oxidase is involved in sensitivity regulation of Sclerotinia sclerotiorum to carbendazim, the influences of this fungicide on mycelial growth, respiration and alternative oxidase encoding gene (aox) expression of S. sclerotiorum were determined. It was found that the inhibition of alternative oxidase alleviated the inhibitory effect of carbendazim on the fungal mycelial growth, indicating that alternative oxidase could increase the sensitivity of S. sclerotiorum to carbendazim. Carbendazim showed no immediate impact on the activities of both cytochrome respiratory pathway and alternative respiratory pathway of S. sclerotiorum mycelia. Pre-treatment for 24 h with carbendazim did not affect the transcript level of aox gene of S. sclerotiorum. However, such pre-treatment obviously induced the alternative respiratory pathway activity of S. sclerotiorum mycelia. The results are helpful for deep understanding of the regulation mechanism of fungicidal activity of carbendazim. Furthermore, they suggest that inducers or activators of alternative oxidase should be applied to acquiring a better controlling effect of carbendazim on S. sclerotiorum in agricultural activities.

CLC Number:

S432.4

Liang Wusheng,Xu Ting,Yao Fei,Li Yonghong and Wang Zhengyi. Enhancing Effect of Alternative Oxidase on Sensitivity of Sclerotinia sclerotiorum to Carbendazim[J]. Chinese Agricultural Science Bulletin, 2016, 32(6): 193-197.

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