Abstract: Red beet is a kind of cultivated sugar beet. Because of the function of nutrition and health care and the extensive use in the treatment of cancer, hypertension, high blood lipid and fatigue as well as in food coloring, red beet has received more and more attention. At present, the domestic cultivated red sugar beet is gradually increasing in number. In order to better protect the interests of the breeders as well as red beet growers and for the purpose of rapid identification of red beet variety, the SSR primer was used to construct the fingerprints of 12 varieties of red beet. The results showed that the combination of the three pairs of SSR primers including BVV21, SSD77 and SSD6 can simply realize the identification of 12 varieties of red beet, of which BVV21 can separately identify 3 varieties of red beet including beetroot, Tianyan red No. 1 andS880274.S