Abstract: In order to find a method for rapidly extracting genomic DNA from sugar beet, a PCR instrument was used in combination with an alkaline lysis method with dried seeds, seedlings and kernels of sugar beet as well as dry powder of sugar beet leaf as raw materials, the concentration of extracted DNA was measured by a micro-spectrophotometer, and amplification of extracted DNA was conducted using the SSR primers of sugar beet. The results demonstrated that DNA was detected in all materials, the mean concentration of extracted DNA from dried seeds, seedlings, kernels and leaf dry powder was 432 ng/μL, 197 ng/μL, 158 ng/μL and 448 ng/μL, respectively, and clear bands were amplified in both DNA stock solution and diluted 20 ng/μL working solution by SSR-PCR reaction system. This method is simple and rapid in extracting genomic DNA from sugar beet, and only requires two medicines NaOH and HCl. The extracted DNA can be completely used for SSR- PCR reaction, which provides technical support for the rapid identification of the purity and authenticity of sugar beet varieties.