Abstract: The paper aims to study the correlation between Cu content in Camellia oleifera forest soil and Camellia oleifera fruit by different extraction methods, and analyze the validity of different Cu extraction agents and their effect on fruit Cu content. The authors extracted Cu from Camellia oleifera forest soil by using HCl, ASI, Bray, DTPA and Mehlich3, and measured the Cu content in the extract, shell, seed coat and kernel by ICP-AES, and did the correlation analysis. The results showed that: the extracting ability of different extraction methods was different, and the order was ASI>DTPA>HCl>M3>Bray; the correlations of soil available Cu content measured by different extraction methods reached extremely significant level; the Cu content of oil seeds was the highest, which was 14.267 mg/kg, followed by that of seed coat, the lowest was that of shell; the soil available Cu content measured by the 5 methods was positively correlated with Cu content in fruit shell and seed kernel of Camellia oleifera, and the correlation with Cu content in seed kernel was significant, while the soil available Cu content was negatively correlated with the Cu content in seed coat. The results indicated that the Cu content in soil and fruit measured was lower than that of the national standard, which provided reference for the pollution-free production of Camellia oleifera.