Abstract: To study the effect of exogenous selenium treatment on yield and the content of selenium, nitrogen, phosphorus and potassium of high quality local conventional rice varieties, indica rice variety‘( Thai Xuan No.1’) and japonica rice variety‘( Jing Xuan No.1’) were used as the material, and different concentrations of selenium treatment were carried out at high altitude mountainous area (above 800 m) with completely random design. The results showed that the application of exogenous selenium did not reduce rice yield of the two varieties; with the increase of exogenous selenium, selenium content in milled rice and rice bran increased, the increasing rate of selenium content in milled rice of japonica variety showed a high linear mode with exogenous selenium increased in the range of 0-15 g/hm2, however, under high concentration of selenium treatment, milled rice rate and head rice rate decreased significantly. With the increase of exogenous selenium concentration, the relationship of nitrogen and potassium content in milled rice with selenium turned from extremely significant positive to extremely significant negative. In low selenium concentration condition, appropriately increase the application amount of nitrogen and potassium could promote the absorption and accumulation of selenium, while in high selenium concentration condition, the application amount of nitrogen and potassium should be controlled. Therefore, the study on the application of exogenous selenium in improving quality and selenium accumulation of high quality local rice varieties was feasible.