Construction and Optimization of CDDP-PCR in Beet

doi:10.11924/j.issn.1000-6850.casb17050112

Abstract

Abstract: The study aims to establish the optimal conserved DNA- derived polymorphism (CDDP) amplification system of sugar beet, with the purpose of using CDDP molecular marker technique to carry out the sugar beet varieties fingerprint construction, core collection construction and molecular marker assisted breeding. This experiment optimized the sugar beet CDDP system by using the method of single factor variable and ultimately determined the beet optimum CDDP system with a total volume of 20 μL, containing 10 μL of MIX, 0.5 μL of primers and 0.5 μL of template DNA. 8 varieties of sugar beets were amplified using the optimized reaction system and the results showed that all materials could be amplified out clear and stable bands and the primer’s polymorphism was very abundant. Thus, the optimized system of CDDP in this study is very suitable for beet CDDP-PCR analysis.

CLC Number:

S566.3

References

1. 兴旺, 潘荣, 崔平. 甜菜种质资源叶部性状多样性及聚类分析. 中国农学通报. 2015,31(30):155-161.
2. 刘乃新, 郭晨晨, 孙燕红, 等. 甜菜SCoT-PCR反应体系的建立及优化. 中国农学通报. 2016,32(34):119-122.
3. 王茂芊, 吴则东, 王华忠. 利用SRAP标记分析我国甜菜三大产区骨干材料的遗传多样性. 作物学报. 2011,37(05):811-819.
4. 吴则东, 王茂芊, 吴玉梅, 等. 32个甜菜品种指纹图谱构建与遗传多样性分析. 中国农学通报. 2015,31(12):169-174.
5. 王玉娟, 张彦, 房经贵, 等. 利用基于RAPD标记的MCID法快速鉴定72个葡萄品种. 中国农业科学. 2012,45(14):2913-2922.
6. 昝逢刚, 应雄美, 吴才文, 等. 98份甘蔗种质资源遗传多样性的AFLP分析. 中国农业科学. 2015,48(05):1002-1010.
7. 段永红, 渠云芳, 王铭, 等. 高粱抗丝黑穗病基因SRAP体系优化. 分子植物育种. 2017(02):611-617.
8. 王瑞云, 季煦, 陆平, 等. 利用荧光SSR分析中国糜子遗传多样性. 作物学报. 2017(04):530-548.
9. 李玉阁, 苏亚中, 张大乐, 等. 中国节节麦基于ISSR标记的遗传多样性分析. 麦类作物学报. 2017,37(01):30-39.
10. 宁洽, 刘文国, 杨伟光, 等. SNP标记在玉米研究上的应用进展. 玉米科学. 2017,25(01):57-61.
11. 胡建斌, 张帆, 李贞煌, 等. 黄瓜DAMD反应体系的建立及种质遗传资源研究. 西北植物学报. 2010,30(04):652-658.
12. 李强, 苏二虎, 高聚林, 等. 大豆CDDP分子标记技术体系的优化、验证及引物筛选. 大豆科学. 2013,32(03):310-315.
13. 李田, 郭俊娥, 郑成淑, 等. 菊花品种的遗传多样性分析及CDDP指纹图谱构建. 北京林业大学学报. 2014,36(04):94-101.
14. 李莹莹. 牡丹CDDP分子标记引物筛选及多态性分析. 核农学报. 2013,27(08):1099-1105.
15. 房文秀, 夏秀英, 安利佳, 等. 越橘种质资源的CDDP遗传多样性及聚类分析. 中国农学通报. 2016,32(28):136-143.
16. Collard BCY, Mackill DJ. Conserved DNA-Derived Polymorphism (CDDP): A Simple and Novel Method for Generating DNA Markers in Plants. Plant Molecular Biology Reporter. 2009;27(4):558-562.
17. Talebi R. Comparative Efficiency of Functional Gene-based Markers, Start Codon Targeted Polymorphism (SCoT) and Conserved DNA-derived Polymorphism (CDDP) with ISSR Markers for Diagnostic Fingerprinting in Wheat (Triticum aestivum L.). Cereal Research Communications. 2014(4);.
18. Hajibarat Z, Saidi A, Hajibarat Z, et.al. Characterization of genetic diversity in chickpea using SSR markers, Start Codon Targeted Polymorphism (SCoT) and Conserved DNA-Derived Polymorphism (CDDP). Physiology and Molecular Biology of Plants. 2015,21(3):1-9.
19. 胡根海, 喻树迅. 利用改良的CTAB法提取棉花叶片总RNA. 棉花学报. 2007,19(01):69-70.
20. 罗霆, 杨海霞, 岑华飞, 等. SCoT分子标记在割手密遗传图谱构建中的应用. 植物遗传资源学报. 2013,14(4):704-710.
21. 熊发前, 蒋菁, 钟瑞春, 等. 两种新型目标分子标记技术—CDDP与PAAP. 植物生理学通讯. 2010(09):871-875.

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