Abstract: In order to establish optimal potato tuber regeneration system. The microtubers and field potatoes of ‘Sharpdi’,‘Favorite’ and ‘Kexin No.1’ were used as the test material. The regeneration system was screened with a controlled variable method to obtain the optimal hormone concentration of the medium. And the comparison of adventitious buds differentiation rates of potato tubers from different sources was conducted. The result showed that the optimal callus induction mediums of ‘Sharpdi’ , ‘Favorite’ and ‘Kexin No.1’ were MS + 1 mg/L IAA + 1 mg/L 6-BA + 0.2 mg/L GA3 + 1 mg/L ZT, MS + 1 mg / L IAA + 1 mg / L 6-BA + 0.2 mg / L GA3 + 2 mg/L ZT and MS+1 mg/L IAA + 1 mg/L 6-BA + 0.5 mg/L GA3 + 2 mg/L ZT respectively. The optimal adventitious buds differentiation medium of ‘Sharpdi’ and ‘Favorite’ was MS + 1 mg/L IAA + 1 mg/L 6-BA + 0.5 mg/L GA3 + 2 mg/L ZT; while for ‘Kexin No.1’,the optimal differentiation medium was MS + 1 mg/L IAA + 1 mg/L 6-BA + 0.2 mg/L GA3 + 2 mg/L ZT. The differentiation rate of adventitious buds in microtuber was significantly higher than field potato.