Silkworm-hosted Cordyceps militaris Cultivation in Larvae of Three Guangxi Silkworm Varieties: A Comparative Analysis

doi:10.11924/j.issn.1000-6850.casb19020023

Abstract

Abstract:

The aim is to screen out the silkworm varieties suitable for producing high-quality and high-yield silkworm-hosted Cordyceps militaris, and to provide references for the comprehensive utilization of sericulture industry resources in Guangxi. The most suitable strain was screened out from three different strains of Cordyceps militaris, and inoculated into the larvae of three Guangxi silkworm varieties to cultivate silkworm-hosted Cordyceps militaris. Through investigating the morphology and characteristics of silkworm-hosted Cordyceps militaris and detecting the contents of biological active components, the most suitable silkworm variety for the culture of Cordyceps militaris was selected. The results showed that the silkworm body and the growth of mycelium of strain A were the best and could be used as the best inoculated strain. ‘Liangguang 2 (orthogonal)’ variety had the best morphology and characteristics of silkworm-hosted Cordyceps militaris in three silkworm varieties. The total mass of silkworm-hosted Cordyceps militaris was 1.361 g, the mass of batrytictic silkworm body was 1.028 g, the total number of sporophores was 10.489 and the length of sporophores was 9.411 cm. ‘Liangguang 2 (orthogonal)’ variety also had the highest content of cordycepin and adenosine in silkworm-hosted Cordyceps militaris, which was 4.84 mg/g and 1.79 mg/g, respectively. The contents of cordycepin in the three species of silkworm-hosted Cordyceps militaris pedestal were all higher than that in the sporophore. ‘Liangguang 2 (orthogonal)’ had the highest content of cordycepin in the silkworm-hosted Cordyceps militaris pedestal and sporophore, which was 7.93 mg/g and 4.79 mg/g, respectively. The contents of sporophore in the three species of silkworm-hosted Cordyceps militaris sporophore were all higher than that in the pedestal. ‘Liangguang 2 (orthogonal)’ had the highest content of cordyceps adenosine in the silkworm-hosted Cordyceps militaris pedestal and sporophore, which was 3.51 mg/g and 6.39 mg/g, respectively. The results showed that the Guangxi silkworm variety ‘Liangguang 2 (orthogonal)’ had better silkworm-hosted Cordyceps militaris index and quality, and could be used as the superior resource.

Key words: Guangxi silkworm varieties, silkworm-hosted Cordyceps militaris, cordycepin, cordyceps adenosine, comparative analysis

CLC Number:

S886.9

Wu Jingjing, Zhou Xiaoling, Dong Guiqing, Lu Chunxia, Liang Guiqiu, Chen Zhi, Lu Fei, Mao Hongbin, Shi Weitao. Silkworm-hosted Cordyceps militaris Cultivation in Larvae of Three Guangxi Silkworm Varieties: A Comparative Analysis[J]. Chinese Agricultural Science Bulletin, 2020, 36(8): 105-110.

Figures/Tables 8

References 20

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菌种编号	蚕体僵化率/%	蚕体转色	菌丝长势	出草率/%
A	90.51±2.34^a	好	++++	94.59±3.20^a
B	74.80±1.24^c	好	+++	82.03±0.41^b
C	83.87±1.55^b	好	++++	89.13±1.58^a

菌种编号	蚕体僵化率/%	蚕体转色	菌丝长势	出草率/%
A	90.51±2.34^a	好	++++	94.59±3.20^a
B	74.80±1.24^c	好	+++	82.03±0.41^b
C	83.87±1.55^b	好	++++	89.13±1.58^a

	差异源	平方和	自由度	均方	F	P值
蚕体僵化率	组间	249.071	2	124.535	39.714	0.007
	组内	9.407	3	3.136
	总数	258.478	5
出草率	组间	158.781	2	79.391	18.482	0.021
	组内	12.886	3	4.295
	总数	171.668	5

	差异源	平方和	自由度	均方	F	P值
蚕体僵化率	组间	249.071	2	124.535	39.714	0.007
	组内	9.407	3	3.136
	总数	258.478	5
出草率	组间	158.781	2	79.391	18.482	0.021
	组内	12.886	3	4.295
	总数	171.668	5

家蚕品种编号	蚕虫草总质量/g	僵蚕体质量/g	子实体总数量/根	子实体长度/cm
两广二号（正交）	1.316±0.017^a	1.028±0.030^a	10.489±0.844^a	9.411±0.812^a
两广二号（反交）	1.163±0.047^ab	0.978±0.022^a	7.571±0.302^b	7.718±0.047^b
桂蚕N2（正交）	1.039±0.071^b	0.866±0.042^b	5.373±0.492^c	7.039±0.166^b