Benomyl Stress: Effects on the Microbial Community Structure in the Intestine of Honey Bees

doi:10.11924/j.issn.1000-6850.casb20190900692

Abstract

Abstract:

The objectives of this study are to determine the change rule of the main intestinal microbial community of honeybee under drug stress, which can provide theoretical references for further investigating the physiological function of intestinal symbiotic community and healthy cultivation of honeybee. The worker bees of Apis mellifera ligustica are used as experimental materials. The intestinal microflora structure was investigated by PCR-DGGE analysis before and after adding benomyl. A specific bright band emerged from the gel after PCR based on different variable regions of rDNA, which indicated that bacteria and fungi were resident in bee intestine. Five bands (2 main belts and 3 sub belts) of the V3 region of 16S rDNA of bacteria and three bands (1 main belt and 2 sub belts) of the 18S rDNA V1+V2 region of fungi were separated by PCR-DGGE, which indicated that there were abundant intestinal microorganisms in honeybee intestine. The bands of b, e, f, g gradually weakened or even disappeared, which indicated that the species and amount of bacteria and fungi in the intestines of honeybees decreased, so the benomyl stress had inhibitory effect on intestinal microorganisms. The benomyl stress can cause the imbalance of intestinal micro-ecology of honeybee, which will damage the normal physiological function of the body and seriously affect the health of honeybee.

Key words: bee, intestinal microorganism, microbial community structures, benomyl stress, DGGE technology

CLC Number:

S895

Dai Junjun, Shu Rui, Liu Jian, Zhang Yuping, Zhang Lili, Chen Ming, Zhao Ping, Liu Zhen. Benomyl Stress: Effects on the Microbial Community Structure in the Intestine of Honey Bees[J]. Chinese Agricultural Science Bulletin, 2020, 36(26): 136-140.

Figures/Tables 3

References 27

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