Beta vulgaris Seedlings: Adaptive Mechanism to Drought Stress

doi:10.11924/j.issn.1000-6850.casb2020-0013

Abstract

Abstract:

To study the response mechanism of sugar beet (Beta vulgaris L.) seedlings in the process of drought stress tolerance, ‘780016B/12you’ was chosen and treated with different concentrations (3%, 6%, 9% and 15%) of PEG6000 drought stress, and the seedling growth was restored to measure the changes in related physiological and biochemical data and aim gene expression among different drought stress treatments. The results showed that, with the increase of drought intensity, the degree of shrinkage and wilting of sugar beet seedlings was gradually serious after 24 h stress treatment, along with the decrease of relative water content (3.48%-15.86%) and chlorophyll content (5.60-13.06), while malondialdehyde and proline of leaves accumulated obviously, especially 15% PEG made malondialdehyde and proline reach about 48.23 μmol/L and 201.37 μmol/L, respectively. After stress recovery, the RWC (96.94%), MDA content (17.22 μmol/L) and morphology of sugar beet treated with 3% PEG basically recovered to the normal level; however, with high concentration of PEG, although the relative water content of seedlings in 9% PEG restored to 8.48%, the malondialdehyde content was still as high as 36.46 μmol/L; the proline always maintained at a high level in each treatment group. qPCR demonstrated that the expression of BvGS gene was induced by drought stress in different degrees with 3.85, 4.45, 5.81 and 8.20 times of that of control. Even after stress recovery, although the expression level of BvGS was decreased, it still maintained at a relative high level. Therefore, it could be inferred that sugar beet seedlings could adapt to the adverse reactions of water loss and plasma membrane peroxidation caused by drought stress through adjusting cell water potential, redox level, photosynthesis and osmotic metabolism balance, and might detoxify the oxidative damage induced by drought stress through glutathione synthesis.

Key words: Beta vulgaris, drought stress, malondialdehyde, proline, glutathione synthetase

CLC Number:

S566.3

Zhang Jing, Wang Jinxia, Guo Mengmeng, Ma Longbiao, Xing Wang, Wang Maoqian, Liu Dali. Beta vulgaris Seedlings: Adaptive Mechanism to Drought Stress[J]. Chinese Agricultural Science Bulletin, 2020, 36(32): 1-7.

Figures/Tables 6

References 35

[1]	Basu S, Ramegowda V, Kumar A, et al. Plant adaptation to drought stress[J]. F1000Research, 2016,5(F1000 Faculty Rev):1554. doi: 10.12688/f1000research URL
[2]	Jung B, Ludewig F, Schulz A, et al. Identification of the transporter responsible for sucrose accumulation in sugar beet taproots[J]. Nature Plants, 2015,1:14001. doi: 10.1038/nplants.2014.1 URL pmid: 27246048
[3]	Moosavi S G R, Ramazani S H R, Hemayati S S, et al. Effect of drought stress on root yield and some morpho-physiological traits in different genotypes of sugar beet (Beta vulgaris L.)[J]. Journal of Crop Science and Biotechnology, 2017,20:167-174. doi: 10.1007/s12892-017-0009-0 URL
[4]	Hosseini S A, Réthoré E, Pluchon S, et al. Calcium application enhances drought stress tolerance in sugar beet and promotes plant biomass and beetroot sucrose concentration[J]. International Journal of Molecular Sciences, 2019,20:3777. doi: 10.3390/ijms20153777 URL
[5]	Pastori G M, Foyer C H. Common components, networks, and pathways of cross-tolerance to stress. The central role of "redox" and abscisic acid-mediated controls[J]. Plant Physiology, 2002,129(2):460-8. URL pmid: 12068093
[6]	Ghaffari H, Tadayon M R, Nadeem M, et al. Proline-mediated changes in antioxidant enzymatic activities and the physiology of sugar beet under drought stress[J]. Acta Physiologiae Plantarum, 2019,41:23. doi: 10.1007/s11738-019-2815-z URL
[7]	Anjum S A, Wang L, Farooq M, et al. Methyl jasmonate-induced alteration in lipid peroxidation, antioxidative defense system and yield in soybean under drought[J]. Journal of Agronomy and Crop Science, 2011,197(4):296-301. doi: 10.1111/j.1439-037X.2011.00468.x URL
[8]	Sharma P, Jha A B, Dubey R S, et al. Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions[J]. Journal of Botony, 2012,2012:1-26.
[9]	Farooq M, Wahid A, Kobayashi N, et al. Plant drought stress: effects, mechanisms and management[J]. Agronomy for Sustainable Development, 2009,29:185-212.
[10]	Wedeking R, Mahlein A-K, Steiner U, et al. Osmotic adjustment of young sugar beets (Beta vulgaris) under progressive drought stress and subsequent rewatering assessed by metabolite analysis and infrared thermography[J]. Functional Plant Biology, 2016,44:119-133. doi: 10.1071/FP16112 URL pmid: 32480551
[11]	Alaei S, Mahna N, Hajilou J, et al. The effect of glycine betaine on the alleviation of drought stress in strawberry pant[J]. Ecology, Environment and Conservation, 2016,22(3):1145-1150.
[12]	Per T S, Khan N A, Reddy P S, et al. Approaches in modulating proline metabolism in plants for salt and drought stress tolerance: phytohormones, mineral nutrients and transgenics[J]. Plant Physiology and Biochemistry, 2017,115:126-140. URL pmid: 28364709
[13]	Zhang M, Wang L, Zhang K, et al. Drought-induced responses of organic osmolytes and proline metabolism durng pre-flowering stage in leaves of peanut (Arachis hypogaea L.)[J]. Journal of Integrative Agriculture, 2017,16(10):2197-2205.
[14]	Ding L, Lu Z, Gao L, et al. Is nitrogen a key determinant of water transport and photosynjournal in higher plants upon drought stress[J]. Frontiers in Plant Science, 2018,9:1143. doi: 10.3389/fpls.2018.01143 URL pmid: 30186291
[15]	Borišev M, Borišev I, Župunski M, et al. Drought impact is alleviated in sugar beets (Beta vulgaris L.) by foliar application of fullerenol nanoparticles[J]. PLoS One, 2016,11:e0166248. doi: 10.1371/journal.pone.0166248 URL pmid: 27832171
[16]	Foyer CH, Noctor G. Redox homeostasis and antioxidant signaling: a metabolic interface between stress perception and physiological responses[J]. Plant Cell, 2005,17(7):1866-1875. doi: 10.1105/tpc.105.033589 URL pmid: 15987996
[17]	Sarker U, Oba S. Catalase, superoxide dismutase and ascorbate-glutathione cycle enzymes confer drought tolerance of Amaranthus tricolor[J]. Scientific Reports, 2018,8:16496. doi: 10.1038/s41598-018-34944-0 URL pmid: 30405159
[18]	Szalai G, Kellös T, Galiba G, et al. Glutathione as an antioxidant and regulatory molecule in plants under abiotic stress conditions[J]. Journal of Plant Growth Regulation, 2009,28:66-80. doi: 10.1007/s00344-008-9075-2 URL
[19]	Ahmad N, Malagoli M, Wirtz M, et al. Drought stress in maize causes differential acclimation responses of glutathione and sulfur metabolism in leaves and roots[J]. BMC Plant Biology, 2016,16:247. doi: 10.1186/s12870-016-0940-z URL pmid: 27829370
[20]	Hoffmann C M. Sucrose accumulation in sugar beet under drought stress[J]. Journal of Agronomy and Crop Science, 2010,196(4):243-252.
[21]	Sharp R E, Hsiao T C, Silk W K. Growth of the maize primary root at low water potentials: II. role of growth and deposition of hexose and potassium in osmotic adjustment. Plant Physiology, 1990,93(4):1337-1346.
[22]	王秋红, 周建朝, 王孝纯. 采用SPAD仪进行甜菜氮素营养诊断技术研究[J]. 中国农学通报, 2015,31(36):92-98.
[23]	Vasantha S, Alarmelu S, Hemaprabha G, et al. Evaluation of promising sugarcane genotypes for drought[J]. Sugar Technology, 2005,7:82-83.
[24]	Laxa M, Liebthal M, Telman W, et al. The role of the plant antioxidant system in drought tolerance[J]. Antioxidants (Basel), 2019,8(4):94.
[25]	Pirasteh-Anosheh H, Saed-Moucheshi A, Pakniyat H, et al. Water Stress and Crop Plants: A Sustainable Approach[M]. Volume 1, Wiley Blackwell, 2016 Oxford, UK.
[26]	Blum A. Osmotic adjustment is a prime drought stress adaptive engine in support of plant production[J]. Plant Cell and Environment, 2017,40(1):4-10.
[27]	Silva D D, Kane M E, Beeson R C. Changes in root and shoot growth and biomass partition resulting from different irrigation intervals for Ligustrum japonicum Thunb[J]. Horticultural Science, 2012,47:1634-1640.
[28]	Lü P, Kang M, Jiang X, et al. RhEXPA4, a rose expansin gene, modulates leaf growth and confers drought and salt tolerance to Arabidopsis[J]. Planta, 2013,237(6):1547-1559. doi: 10.1007/s00425-013-1867-3 URL pmid: 23503758
[29]	Zhang J Y, Cruz D E Carvalho M H, et al. Global reprogramming of transcription and metabolism in Medicago truncatula during progressive drought and after rewatering[J]. Plant Cell and Environment, 2014,37(11):2553-2576.
[30]	He F, Sheng M, Tang M. Effects of Rhizophagus irregularis on photosynjournal and antioxidative enzymatic system in Robinia pseudoacacia L. under drought stress[J]. Frontiers in Plant Science, 2017,8:183. doi: 10.3389/fpls.2017.00183 URL pmid: 28261240
[31]	Sales C R G, Ribeiro R V, Silveira J A G, et al. Superoxide dismutase and ascorbate peroxidase improve the recovery of photosynjournal in sugarcane plants subjected to water deficit and low substrate temperature[J]. Plant Physiology and Biochemistry, 2013,73:326-336. URL pmid: 24184453
[32]	Upreti K K, Murti G S R, Bhatt RM. Response of pea cultivars to water stress: changes in morpho-physiological characters, endogenous hormones and yield[J]. Journal of Vegetation Science, 2000,27:57-61.
[33]	Wahid A, Rasul E. Photosynthesis in leaf, stem, flower and fruit[M], in: Pessarakli M. (Ed.), Handbook of Photosynthesis, 2nd ed., CRC Press, Florida, 2005: 479-497.
[34]	Anjum F, Yaseen M, Rasul E, et al. Water stress in barley (Hordeum vulgare L.). I. Effect on chemical composition and chlorophyll contents[J]. Pakistan Journal of Agricultural Science, 2003,40:45-49.
[35]	Kavar T, Maras M, Kidric M, et al. Identification of genes involved in the response of leaves of Phaseolus vulgaris to drought stress[J]. Molecular Breeding, 2007,21:159-172.

PEG	RWC/%
PEG	0 h	24 h	48 h (复性)	72 h (复性)
3%	96.74±3.29	93.26±3.78	93.20±4.65	96.94±5.73
6%	96.70±3.07	90.04±4.05	93.27±4.91	96.78±6.25
9%	97.83±3.81	84.33±5.72	87.88±5.03	89.35±5.87
15%	96.58±3.56	80.72±5.34	74.08±4.34	71.91±6.71

PEG	RWC/%
PEG	0 h	24 h	48 h (复性)	72 h (复性)
3%	96.74±3.29	93.26±3.78	93.20±4.65	96.94±5.73
6%	96.70±3.07	90.04±4.05	93.27±4.91	96.78±6.25
9%	97.83±3.81	84.33±5.72	87.88±5.03	89.35±5.87
15%	96.58±3.56	80.72±5.34	74.08±4.34	71.91±6.71